主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (9): 611-615.doi: 10.3969/j.issn.1006-2157.2014.09.009

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Influences of nanoparticles Rg3 and PEG-PLGA-Rg3 on endothelial cells invasion and tube formation

GENG Liang1, YANG Cai-ling2, TIAN Tong-de1, YU Jing3, SUN Wei-min4, JIN Xi-yuan4, HUA Bao-jin5   

  1. 1 Oncology Hospital, Zhengzhou University, Henan 450008;
    2 First Affiliated Hospital of Xinxiang Medical College;
    3 Beijing Friendship Hospital, Capital University of Medical Sciences;
    4 Institute of Life Sciences and Bioengineering, Beijing Jiaotong University;
    5 Guang’anmen Hospital, China Academy of Chinese Medical Sciences
  • Received:2014-03-04 Online:2014-09-30 Published:2014-09-30

Abstract: Objective To observe the influences of ginsenoside Rg3 (Rg3) and PEG-PLGA-Rg3 slow-release nanoparticles (Rg3-N) on endothelial cells invasion and tube formation in vitro, and compared the difference. Methods The influences of Rg3 and PEG-PLGA Rg3-N on endothelial cells invasion and tube formation were observed by using transwell method and tube formation method, and the difference between effects of Rg3 and PEG-PLGA Rg3-N were compared. The gene and protein expressions of E-cadherins (E-cad), matrix metalloproteinase-9 (MMP-9), hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) were detected by using RT-PCR and Western blot method for exploring the internal molecular mechanism. Results The treatment with Rg3 and PEG-PLGA Rg3-N reduced significantly the invasive ability and tube formation of human EA.hy926 endothelial cells (P<0.05), had no significant influences on protein and gene expressions of VEGF, and decreased the expressions of MMP-9 and E-cad. There was no protein expression of HIF-1α observed in all groups. Conclusion The slow-release Rg3 and PEG-PLGA Rg3-N can inhibit the invasive ability and tube formation of human EA.hy926 endothelial cells through inhibiting the expressions of E-cad and MMP-9. PEG-PLGA Rg3-N can improve the antagonism ability to invasion and tube formation of human EA.hy926 endothelial cells in vitro.

Key words: ginsenoside Rg3, nanoparticles, vascular endothelial cells, tumor angiogenesis

CLC Number: 

  • R285.5