主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (9): 616-619.doi: 10.3969/j.issn.1006-2157.2014.09.010

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Influence of therapy of coordinating heart and kidney on vascular endothelial L-Arg/NOS/NO pathway in aged female rats

XIN Ming-wei1,2, LIU Yan-feng1, LUO Bin3, WU Ying2, JIANG Mei3, SUN Tian-lin3   

  1. 1 Dongzhimen Hospital, Beijing University of Chinese Medicine,Beijing 100700;
    2 Beijing Obstetrics and Gynecology Hospital, Capital University of Medical Sciences;
    3 Beijing University of Chinese Medicine
  • Received:2014-03-16 Online:2014-09-30 Published:2014-09-30

Abstract: Objective To investigate the influence of therapy of coordinating heart and kidney on vascular endothelial L-Arg/NOS/NO pathway in aged female rats. Methods Female SD rats (15 months old) were divided into aged group, Chinese medicinal group(20.65 g/kg), Western drug control group(0.12 mg/kg) and Chinese medicinal control group(0.65 g/kg), and respectively given orally normal saline (NS), Chinese medicinal of coordinating heart and kidney, Bujiale and Gengnian’an. Other rats (3 months old) were chosen as young group. The levels of serum estradiol (E2), follicular stimulating hormone (FSH) and luteinizing hormone (LH) were detected by using radioimmunoassay, level of aontic tunica. wdreritita nitric oxide (NO) was detected by using nitrate reductase method and levels of aortic tunica adventitia nitric oxide synthase (NOS) and L-Arg were detected by using colorimetric method. Results Compared with aged group, NOS level had no significant changes in Chinese medicinal group (P>0.05), decrased significantly in Chinese medicinal control group and Western drug control group (P<0.05). Compared with aged group, L-Arg level had no significant changes in Chinese medicinal group and Western drug control group (P>0.05), decreased in Chinese medicinal control group (P<0.05). Compared with aged group, NO level increased in Chinese medicinal group and Western drug control group (P<0.05), and slightly increased in Chinese medicinal control group (P>0.05). The level of estrogen was correlated to NO level. Conclusion The therapy of coordinating heart and kidney can improve estrogen level and has regulating effect on vascular endothelial L-Arg/NOS/NO pathway in aged female rats.

Key words: therapy of coordinating heart and kidney, aged female rats, sex hormones level, vascular endothelial L-Arg/NOS/NO pathway

CLC Number: 

  • R285.5