主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (11): 767-772.doi: 10.3969/j.issn.1006-2157.2014.11.011

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Effects and mechanism of peppermint oil on membrane fluidity and potentials of HaCaT keratinocytes

LAN Yi, CHEN Yan-yan, ZHAO Bo-chen, LIU Na, WANG Wen-ping, ZHANG Qing, WU Qing   

  1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102
  • Received:2014-05-21 Online:2014-11-30 Published:2014-11-30

Abstract: Objective To observe the effects of peppermint oil on membrane fluidity and membrane potentials of HaCaT keratinocytes, so as to reveal the potential mechanism of enhancing transdermal penetration. Methods The cytotoxicity of peppermint oil was evaluated by using cell counting kit (CCK-8) assay. The fluorescence recovery after photobleaching (FRAP) technique was adopted to measure the change of membrane fluidity of HaCaT cells. The flow cytometer was used to study the alteration of membrane potentials of HaCaT cells, and to determine the intracellular concentration of Ca2+. Ca2+-ATPase activity in HaCaT cells was detected by using Ca2+-ATPase Kit. Results Compared with azone, a common chemical penetration enhancer, peppermint oil extracted from Mentha haplocalyx Briq. had lower cytotoxicity. Peppermint oil increased HaCaT cells membrane fluidity and decreased membrane potentials. Meanwhile, the intracellular concentration of Ca2+ was increased significantly, and the Ca2+-ATPase activity was decreased (P<0.05). Conclusion Peppermint oil influenced the membrane fluidity and potentials of cells by breaking the Ca2+ balance of the inner and outer membrane, leading to the higher skin permeability to enhance the transdermal absorption of drugs.

Key words: peppermint oil, HaCaT, membrane fluidity, membrane potential, Ca2+, penetration enhancer

CLC Number: 

  • R285.5