主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (1): 37-41.doi: 10.3969/j.issn.1006-2157.2015.01.009

Previous Articles     Next Articles

Preventive and therapeutic effects of Fuzheng Tixie Souluo formula on pulmonary interstitial fibrosis rats

YAN Yu-qin,ZHANG Li-shan,WU Hua-yang,WANG Chen,WANG Lin-yang,ZHOU Shao-zhong,GUAN Qiu-hong,LI De-ying,SU Hui-ping   

  1. Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700
  • Received:2013-09-06 Online:2015-01-30 Published:2015-01-30

Abstract: Objective To explore the preventive and therapeutic effects of Fuzheng Tixie Souluo formula, which was formulated by TCM theory of collateral disease , on rat model with pulmonary interstitial fibrosis induced by bleomycin at different time points. Methods SD rats were divided into sham-operation group, model group, hydrocortisone group and Fuzheng Tixie Souluo formula group (Chinese medicine group) randomly. Rats were infused bleomycin through tracheal intubation to establish pulmonary fibrosis model except those in sham-operation group which were administered intragestrically the same volume of normal saline. From 24 h after modeling, sham-operation group and model group were given saline, hydrocortisone group and Chinese medicine group were given corresponding drugs. Rats were sacrificed at 7, 14, 28, 45 d respectively,with the lung tissues were collected. Pathological changes of the lungs were examined by HE and Masson staining, content of transformation growth factor-β1(TGF-β1) was tested by ELISA, expression of Smad3,Smad4 and Smad7 protein in the lung tissues were evaluated by RT-PCR. Results Compared with model group,the degree of alveolitis at the 7th day,14th day and the degree of fibrosis at the 14th day, 28th day and 45th day in Chinese medicine group decreased remarkably(P<0.05).TGF-β1 expression in Chinese medicine group was lower than that in model group at each time points. The expressions of Smad3 and Smad4 in Chinese medicine group decreased and Smad7 increased significantly(P<0.01). Conclusion Fuzheng Tixie Souluo formula not only can prevent the formation of alveolitis and fibrosis,but also delay the progress of pulmonary fibrosis induced by bleomycin over long period.The potential mechanisms may be related to the signal transduction of TGF-β1, attributed to the upregulation of the expression of Smad3 /Smad 4 and downregulation of the expression of Smad7.

Key words: pulmonary interstitial fibrosis, Fuzheng Tixie Souluo formula, Smad, transformation growth factor-β1, different time points, rats

CLC Number: 

  • R285.5