主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (2): 85-88.doi: 10.3969/j.issn.1006-2157.2015.02.004

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Effects of paeoniflorin on histomorphology and NO pathway in hippocampus CA3 Zone in a rat model of blood deficiency and liver depression pattern*

WANG Jing-xia1, ZHANG Jian-jun#1, QU Sheng-sheng1, WANG Chun1, LI Yan-xia1, GE Yang1,JIANG Ye2, RAN Cong-cong2   

  1. 1 Beijing University of Chinese Medicine, 10029;
    2.Hebei Medical University
  • Received:2014-07-08 Online:2015-02-28 Published:2015-02-28

Abstract: Objective To investigate the effects of paeoniflorin on histomorphology, Glu and NO levels in hippocampus, and on protein expressions of iNOS in the hippocampus CA3 Zone in a rat model of blood deficiency and liver depression pattern. Methods The rat model of blood deficiency and liver depression pattern was established with chronic restraint stress in conjunction with radiation. Fifty-six male SD rats were randomly divided into model group, high-dose, medium-dose, and low-dose paeoniflorin groups, as well as the blank control group of healthy SD rats(n=14 in each group). SD rats were treated with intragastric administration of paeoniflorin in the high-dose, medium-dose, and low-dose paeoniflorin groups while pure water was intragastrically administered in the model and blank groups for 21 days. Histopathological changes of hippocampus CA3 Zone were observed by using HE staining methods, and Glu and NO levels in hippocampus tissues was measured by using HPLC and the Griess methods, respectively, and protein expression of iNOS in hippocampus CA3 zone was detected with immunohistochemistry. Results Compared with the blank group, significant changes were observed in the neuron cells in the model group, including cell body shrinkage, deeply stained chromatin nuclei, triangular nuclear shrinkage and no remarkable nucleoli. There was no significant changes in paeoniflorin group. The levels of Glu and NO increased significantly in model group compared with normal group (P<0.05), and decreased significantly in paeoniflorin group compared with model group (P<0.05). The protein expression of iNOS in hippocampus CA3 zone increased significantly in the model group compared with the blank control group (P<0.05), and decreased significantly in paeoniflorin group compared with the model group (P<0.05). Conclusion Pathological changes including neuron shrinkage were present in hippocampus CA3 Zone in the model of blood deficiency and liver depression pattern. Paeoniflorin could improve hippocampus functional structural damage in the rat model of blood deficiency and liver depression pattern, which may be related to decreased levels of Glu and NO and protein expression of iNOS in hippocampus CA3 Zone.

Key words: blood deficiency and liver depression pattern, NO, iNOS, paeoniflorin

CLC Number: 

  • R285.5