主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (2): 89-94.doi: 10.3969/j.issn.1006-2157.2015.02.005

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Effects of blood-cooling and blood-activating medicinal on IL-23/IL-17 axis in psoriasiform mice model induced by imiquimod*

ZHAO Jing-xia, DI Ting-ting, WANG Yan, LIU Xin,LIANG Dai-ying,LI Ping#   

  1. Beijing Institute of Traditional Chinese Medicine, Beijing Hospital of Traditional Chinese Medicine affiliated to Capital Medical University, Beijing 100010
  • Received:2014-07-05 Online:2015-02-28 Published:2015-02-28

Abstract: Objective To observe the effects of blood-cooling and blood-activating medicinal (Liang Xue Huo Xue Yao) on IL-23/IL-17 axis in psoriasiform mice model induced by imiquimod. Methods BALB/c male mice were randomly divided into control group, model group, and three blood-cooling and blood-activating medicinal groups of high, medium and low dose (LXHX-H, LXHX-M and LXHX-L groups) respectively. Pathological changes of skin lesions in mice were observed by using light microscope. The levels, mRNA and protein expression of the cytokines in the serum and skin lesions of mice were tested by using ELISA, RT-PCR, and western blotting assay respectively. Results Compared with model group, LXHX-H, LXHX-M and LXHX-L groups decreased the emergence, color change and infiltration of skin erythema, as well as the PASI scores, epidermal parakeratosis and epidermal over-proliferation of the mice. According to the trend graph of the PASI scores, the area under the curve of LXHX-M group was significantly lower than that in model group. HE staining showed that, compared with model group, mice in LXHX groups had more smooth epidermis cortex, less epidermal parakeratosis and decreased epidermal over-proliferation(P<0.01). Lesions protein and mRNA test showed that compared with model group, mice in LXHX groups had lower expression level of IL-17 and IL-23 protein, as well as IL-23mRNA, IL-22mRNA, and RORγt mRNA(P<0.01 or P<0.05). Conclusion LXHX medicinal could significantly decrease the intensity of the psoriasiform inflammation and the formation of psoriasiform skin lesions induced by imiquimod, the effect of which might be related to the decrease of IL-23/IL-17 axis cytokine protein and the expression of mRNA, as well as the inhibition of the expression of Th17 cell differentiation transcription factor RORγt mRNA.

Key words: psoriasis, IL-23/IL-17 axis, blood-cooling and blood-activating medicinal (Liang Xue Huo Xue Yao), mice

CLC Number: 

  • 285.5