主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (2): 100-103.doi: 10.3969/j.issn.1006-2157.2015.02.007

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Effects of Danzhi Xiaoyao San on cell apoptosis of Papez’s circuit in rat model of Generalized Anxiety Disease (GAD)*

DONG Ning1, TANG Qi-sheng2#, ZHAO Rui-zhen2, XU Shuo1, YANG Xin-ke2, MAO Ying-qiu1   

  1. 1 Beijing University of Chinese Medicine, Beijing 100029;
    2 The Third Affiliated Hospital, Beijing University of Chinese Medicine
  • Received:2014-07-17 Online:2015-02-28 Published:2015-02-28

Abstract: Objective To study the effects of Danzhi Xiaoyao San on cell apoptosis of Papez’s circuit in rat model of Generalized Anxiety Disease (GAD). Methods Male Wistar rats were divided into the normal group, model group, Chinese Medicine group, and control group of western medicine; each group contained 10 rats. The GAD model was established by using randomly assigned empty drinking bottle to upset the rats. Then the Chinese medicine group was intragastrically given Danzhi Xiaoyan San, while the western medicine group was given buspirone hydrochloride, and the model group was given distilled water once a day for a week. Then morphological changes of hippocampus and amygdaloid nucleus were observed by using light and electron microscopes, and the early apoptosis rate of hippocampus and mamillary body were evaluated by applying flow cytometry assays after removing rat brain. Results Cells in hippocampus and amygdaloid nucleus of model group showed typical morphological changes of apoptosis such as margination and karyopyknosis. Danzhi Xiaoyao San and buspirone hydrochloride could revert the changes markedly. Flow cytometry assay showed that the early apoptosis rates in hippocampus and mamillary body of model group increased significantly compared with normal group (P<0.01). Compared with model group, the apoptosis rates of Chinese medicine group and Western medicine group decreased (P<0.05 or P<0.01). Conclusion There is extensive and obvious apoptosis in Papez’s circuit of GAD rat’s brain. Danzhi Xiaoyao San can prevent and inhibit apoptosis to some extent.

Key words: Danzhi Xiaoyao San, Generalized Anxiety Disease, Papez’s circuit, apoptosis

CLC Number: 

  • R285.5