主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (2): 104-110.doi: 10.3969/j.issn.1006-2157.2015.02.008

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Effects of Blood Mansion Stasis-Expelling Decoction combined with Two Matured Substances Decoction on adipocytokines in obese rats*

YANG Shi-quan1, CHEN Gui-hai1,2#, DENG Wei3, CHEN Huan-xin3, CHEN Juan4, JIANG Xiao1, ZANG Zhi-ming1   

  1. 1 Guangxi University of Traditional Chinese Medicine, Guangxi Nanning 530001;
    2 Shaanxi University of Chinese Medicine;
    3 The Second Traditional Chinese Medicine Hospital of Kaifeng;
    4 Beijing Technology Garden Xinhai Medicine Company
  • Received:2013-12-27 Online:2015-02-28 Published:2015-02-28

Abstract: Objective To investigate the effects of Blood Mansion Stasis-Expelling Decoction (Xuefu Zhuyu Tang) in combination with Two Matured Substances Decoction (Er Chen Tang) on adipocytokines in subcutaneous adipose tissues, visceral adipose tissues and serum of obese rats. Methods Nutritional obesity rat models were established with high-fat feeding, and they were randomly divided into four groups according to body mass, namely, Blood Mansion Stasis-Expelling Decoction group, Yang-Supplementing and Five-Returning Decoction group (Buyang Huanwu Tang), Blood Mansion Stasis-Expelling Decoction in combination with Two Matured Substances Decoction group, model group plus blank control group (n=10). The first three groups were administered with Blood Mansion Stasis-Expelling Decoction, Yang-Supplementing and Five-Returning Decoction and Blood Mansion Stasis-Expelling Decoction in combination with Two Matured Substances Decoction, respectively at 10 times the adult dosage. The latter two groups were fed with saline. All the groups were intervened for 4 consecutive weeks. The levels of subcutaneous adipose tissue, visceral adipose tissue, serum of visceral fat element, adiponectin, leptin, fibrinolytic enzyme activators inhibitor-1, tumor necrosis factor alpha, and endothelin-1 (plasma) were measured by using Elisa method. Results ①The visfatin levels in subcutaneous fat, visceral fat and serum were increased in the model group, which were lowered in the Blood Mansion Stasis-Expelling Decoction group and the Blood Mansion Stasis-Expelling Decoction in combination with Two Matured Substances Decoction group compared with the model group (P<0.05), more significant in the latter (P<0.05). ②The contents of subcutaneous fat, visceral fat and serum adiponectin were reduced in the model group,while the contents of subcutaneous adipose tissue and serum adiponectin in every blood-activating group were increased compared with that of model group (P<0.05), more significant in Yang-Supplementing and Five-Returning Decoction group (P<0.05). Moreover, the level of adiponectin in visceral adipose tissue in Yang-Supplementing and Five-Returning Decoction group was also higher than that in model group(P<0.05). ③The level of leptin was decreased in subcutaneous fat and increased in visceral fat and in serum in model group(P<0.05). Serum leptin levels were lowered in three blood-activating groups. Moreover, leptin in visceral adipose tissue was also lowered in Blood Mansion Stasis-Expelling Decoction in combination with Two Matured Substances Decoction group (P<0.05).④The level of TNF alpha in visceral fat was reduced (P<0.05), No significant difference was observed in serum concentration and content of TNF alpha in subcutaneous fat between groups(P>0.05). Compared with model group, levels of TNF alpha in visceral fat were elevated in Yang-Supplementing and Five-Returning Decoction group and Blood Mansion Stasis-Expelling Decoction in combination with Two Matured Substances Decoction group (P<0.05). ⑤No statistically significant differences were shown in endothelin-1 in subcutaneous fat, visceral fat and plasma (P>0.05). ⑥The levels of PAI-1 in visceral fat and subcutaneous fat were increased in model group (P<0.05). However, PAI-1 level in visceral fat was reduced in Blood Mansion Stasis-Expelling Decoction group, and Blood Mansion Stasis-Expelling Decoction in combination with Two Matured Substances Decoction group (P<0.05). In addition, the level of PAI-1 in subcutaneous fat tissues was also lowered (P<0.05). Conclusion There was a significant endocrinological difference between visceral and subcutaneous adipose tissue. For model rats, formulas that can activate the blood and resolve blood stasis have therapeutical effects on disorder of adipocytokines.

Key words: nutritional obesity, adipocytokines, activating blood and removing stasis

CLC Number: 

  • R285.5