主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (2): 115-119.doi: 10.3969/j.issn.1006-2157.2015.02.010

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Effects of saikosaponina on the behaviors and expressions of Caspase-3 and Caspase-9 in hippocampus of rats with depression*

CAI Zhen-zhen1, XU Guang-you2, WEN Qiu-ting2, WANG Shao-qing2, ZHANG Xiao-jie2, ZHANG Jing-yan2#   

  1. 1 Mental Health Institute of Qiqihar Medical College, Heilongjiang 161006;
    2 Pathology Department of Qiqihar Medical College
  • Received:2014-07-15 Online:2015-02-28 Published:2015-02-28

Abstract: Objective To investigate the effects of saikosaponina on the behaviors and expressions of Caspase-3 and Caspase-9 in hippocampus of rats with depression. Methods SD rats (2-3 months old, n=60) were randomly divided into control group, model group, saikosaponina group, and fluoxetine group (n=15 in each group). The model of depression was established by using chronic unpredictable mild stress (CUMS) combined with isolated rearing. The fluoxetine group and saikosaponina group were given intragastric administration of fluoxetine at 1.2 mg/Kg or saikosaponina at 25 mg/Kg once daily from the second day of the establishment of the model. The sucrose consumption experiment and open field test were carried out to assess rat ethological changes on the 21st day. The protein and mRNA expressions of Caspase-3 and Caspase-9 in rat hippocampus were detected by using immunohistochemical technique and RT-PCR. Results Ethological parameters decreased significantly in model group (P<0.05) at Day 21 after the establishment of the model, compared with control group. Compared with model group, they were significantly improved in fluoxetine and saikosaponina group (P<0.05). The results of immunohistochemistry and PCR test showed that the expressions of Caspase-3, Caspase-9 protein, and mRNA increased in model group compared with control group (P<0.05), and decreased in fluoxetine group and saikosaponina group (P<0.05). Conclusion Saikosaponina can effectively relieve the symptoms of depression in rats, and its mechanism may be related to the decrease of the expressions of Caspase-3 and Caspase-9 in hippocampus.

Key words: saikosaponina, chronic unpredictable stress, depression, Caspase-3, Caspase-9

CLC Number: 

  • R285.5