主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (2): 120-125.doi: 10.3969/j.issn.1006-2157.2015.02.011

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Qingdu Huoxue Huatan Compound inhibits the expression of nf-kappa B and STAT3 in umbilical vein endothelial cell line ECV304 OGD/R model*

LUO Shu, SHAO Jia#, WU Hao-xin, CHEN Gang, LIU Zhou   

  1. Nanjing University of Traditional Chinese medicine, Nanjing 210046
  • Received:2014-08-12 Online:2015-02-28 Published:2015-02-28

Abstract: Objective To study the protective effects of Qingdu Huoxue Huatan (Toxin-removing blood-activating and phlegm-resolving) compound against OGD/R damage to umbilical vein endothelial cell and its underlying mechanism. Methods SD rats were intragastrically given Qingdu Huoxue Huatan compound (12 g/kg) while the control group received normal saline of the same dosage. Sera of rats in two groups were extracted afterwards. Drug serum of Qindu Huoxue Huatan compound was added to the OGD/R-damaged umbilical vein endothelial cell line ECV304 in the process of regainning oxygen and sugar, and cell survival rate was evaluated by using MTT and mRNA; protein levels of NF-κB and STAT3 were measured by using rt-PCR and western blotting. Results After 24h, Qindu Huoxue Huatan compound drug serum promoted ECV304 proliferation, and the promotion reached the peak at 48 h. Inhibition of mRNA and protein of NF-κB and STAT3 was observed at each time period. Conclusion Qindu Huoxue Huatan compound drug serum could effectively improve OGD/R damage to umbilical vein endothelial cell line ECV304, and promote cell’s proliferation. Its mechanism may be related to the inhibited expressions of nf-kappa B and STAT3.

Key words: Qindu Huoxue Huatan compound, umbilical vein endothelial cell, OGD/R, NF-κB, STAT3, rat

CLC Number: 

  • R285.5