主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (2): 126-129.doi: 10.3969/j.issn.1006-2157.2015.02.012

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Synthesis and immunogenicity identification of artificial antigen of Saikosaponin a*

FENG Hui-bin,ZHAO Yan,ZHAO Ling-ling,SHAN Wen-chao, ZHAO Yan,ZHANG Yue,WANG Qing-guo, QU Hui-hua#   

  1. Beijing University of Chinese Medicine, Beijing 100029
  • Received:2014-03-19 Online:2015-02-28 Published:2015-02-28

Abstract: Objective To establish an enzyme linked immunosorbent assay (ELISA) for Saikosaponin a on the basis of the synthesis of immunizing antigen and coating antigen of Saikosaponin. Method The method of Sodium Permanganate Oxidation was used to synthesize immunizing antigen (SSa-BSA) and coating antigen (SSa-PLL) of Saikosaponin a separately; the success of the synthesis of artificial antigens was examined by using UV spectrometry and TLC (thin layer chromatography) method. BALB/c mice were immunized with the prepared SSa-BSA immunogenic antigen. The titer and specificity of the anti-serum were detected with ELISA and indirect competitive ELISA(ic-ELISA) respectively. Results The results of TLC showed that Saikosaponin a was successfully conjugated with BSA and PLL. After immunized with SSa-BSA, the mice can produce specific anti-saikosaponina antibodies, whose titer was up to 1:80000. Conclusion The research results showed that TLC had a unique advantage over other artificial antigen identification methods. Artificial antigen of Saikosaponin a was successfully synthesized, which could be applied to the preparation of monoclonal antibodies, as well as the establishment of corresponding immune method.

Key words: Saikosaponin a, artificial antigen, sodium periodate oxidation, immunoassay method, anti-saikosaponin a antibodies

CLC Number: 

  • R284.1