主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (3): 167-171.doi: 10.3969/j.issn.1006-2157.2015.03.005

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Effect of curcumin on expression of IRS-1 of double transgenic mice model with Alzheimer’s disease*

DANG Hui-zi 1, WEI Peng1,2, CHEN Xiao-pei1,3, REN Ying1, YANG Jin-duo1, WANG Peng-wen1#   

  1. 1 Laboratory of Pharmacology of Dongzhimen Hospital ,Beijing University of Chinese Medicine, Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Beijing 100700;
    2 Jiaozuo Hospital of TCM, Henan Province;
    3 Kaifeng Hospital of TCM, Henan Province
  • Received:2014-09-23 Online:2015-03-28 Published:2015-03-28

Abstract: Objective To observe the effect of curcumin on the expression of insulin receptor substrate-1 (IRS-1) and phosphated insulin receptor substrate-1( p-IRS-1) in the hippocampus of mice model with Alzheimer’s disease (AD), so as to explore the mechanism of neuro-protective effect of curcumin. Methods Three-month-old APP/PS1 double transgenic mice were randomly divided into model group, rosiglitazone group(10 mg/kg) and high-, mid-, low- dose curcumin groups(high-dose group, mid-dose group, low-dose group)with the dose of 400, 200 and 100 mg/kg (n=12 each). Non-transgenic mice of the same brood were selected as control group. After intragastric administration for six consecutive months, the protein expressions of IRS-1 and p-IRS-1 in hippocampus CA1 area were detected by immunohistochemistry and Western blot,and the mRNA expression of IRS-1 were detected by RT-PCR. Results IRS-1 positive cells of hippocampus CA1 area significantly increased in model group than those of normal group (P<0.01). Compared with model group, IRS-1 positive cells decreased(P<0.05 or P<0.01)significantly in rosiglitazone group and three curcumin groups, while the expression of p-IRS-1 increased in all four intervention groups(P<0.05 or P<0.01). Conclusion Curcumin can recover, to some extent, the protein contents of IRS-1 and p-IRS-1 in hippocampus CA1 area of APP/PS1 double transgenic mice. Furthermore, the expression of IRS-1mRNA can be down-regulated and insulin-signaling transmission of the APP/PS1 double transgenic mice can be improved. All these findings suggest that the potential mechanism of anti-AD effect of curcumin is by the way of regulating insulin signal transduction pathway.

Key words: curcumin, Alzheimer’s disease, double transgenic mice, IRS-1, p-IRS-1

CLC Number: 

  • R285.5