主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (3): 182-185.doi: 10.3969/j.issn.1006-2157.2015.03.008

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Effect of antidepression and mechanism of regulation on IDO of Tiansi liquid*

ZHOU Jing-yang1,LU Yi1,XU Xiang-qing2, ZHANG Jing1,LI Hai-na1, CHANG Hong-sheng1#   

  1. 1 Beijing University of Chinese Medicine, Beijing 100029;
    2 Affiliated Hospital of Shandong University of Chinese Medicine
  • Received:2014-10-13 Online:2015-03-28 Published:2015-03-28

Abstract: Objective To study the effect of antidepression of Tiansi Liquid,a TCM basic prescription for treating amnesia and palpipation, which influences on indoleamine 2, 3-dioxygenase (IDO), the key metabolism enzyme of the tryptophan-kynurenine pathway. Methods The mice model of depression was established by intraperitoneal injection of 1 mg/kg lipopolysaccharide (LPS). The immobility time in forced swimming test was collected by Noldus EthoVision XT9 system. The content of tryptophan and kynurenine in brain tissue were detected by HPLC-MS/MS technique. The mRNA expression level of IDO and NF-κB in brain tissue were measured by using real-time PCR. Results Four hours after the injection of LPS in model group, the immobility time of mice was prolonged (P<0.05), while the activity of IDO in brain tissue and the mRNA expression level of IDO and NF-κB increased significantly(P<0.01). Tiansi liquid shortened the immobility time of mice (P<0.05) , and inhibited the activity of IDO and the expression of IDO and NF-κB mRNA (P<0.01). Conclusion Antidepressant-like drug effect of Tiansi liquid is due to inhabiting directly and indirectly effects of NF-κB on IDO which is activated by inflammatory cytokines.

Key words: Tiansi liquid, anti-depression, indoleamine2, 3-dioxygenase (IDO), mice

CLC Number: 

  • R285.5