主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (3): 190-194.doi: 10.3969/j.issn.1006-2157.2015.03.010

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Effects of Yiqi Huoxue formula and Bushen Shengsui formula on expression of Wnt3a,Frizzled9 and TCF3 in hippocampus of rats with ischemia-reperfusion injury*

SONG Zu-rong1,WANG Jian2,HU Jian-peng2#,XU Wei2   

  1. 1 College of Pharmacy, Anhui Xinhua University,Anhui 230088;
    2 Key Laboratory of Xin’an Medicine Jointly Developed by Ministry of Education and Anhui Province, Anhui University of Traditional Chinese Medicine
  • Received:2014-09-23 Online:2015-03-28 Published:2015-03-28

Abstract: Objective To study the effects of Yiqi Huoxue formula (YQHX) and Bushen Shengsui formula (BSSS) on the protein expression of Wnt3a,Frizzled 9 and TCF3 on Wnt/β-catenin pathway in hippocampus of rat model induced by middle cerebral artery ischemia/reperfusion. Methods The focal cerebral ischemia/ reperfusion rat model was established by intraluminal thread occlusion of the middle cerebral artery (MCAO)for 2-h followed by 7-d reperfusion. The rats were randomly divided into sham group, model group, YQHX group and BSSS group(each n=10). The expression of Wnt3a, Frizzled9 and TCF3 protein in hippocampus of cerebral ischemia were detected by Western-Blot assay,and those in CA1 zone of cerebral ischemia were measured by using immunohistochemistry Envision two-step method. Results The relative expression of Wnt3a,Frizzled 9 and TCF3 protein in both hippocamous and CA1 zone increased significantly in model group compared with sham group (P<0.01). Compared with model group, the expression of those three proteins in both hippocamous and CA1 zone decreased significantly in YQHX group and BSSS group (P<0.05). Conclusion Yiqi Huoxue formula and Bushen Shengsui formula can promote the repairment of injured cerebral tissue by the way of reducing the protein expression of Wnt3a,Frizzled9 and TCF3 in hippocampus to regulate Wnt/β-catenin signal pathway.

Key words: cerebral ischemia-reperfusion injury, Yiqi Huoxue formula, Bushen Shengsui formula, hippocampus, Wnt3a, Frizzled9, TCF3, rats

CLC Number: 

  • R285.5