主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (3): 201-205.doi: 10.3969/j.issn.1006-2157.2015.03.012

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Fingerprints for characterization of nine compatibility formula and optimization of compatibility formula engaged in activating blood and resolving stasis :a study for Sanhuang Xiexin decoction composed of Radix et Rhizoma rhei, Radix scutellariae and Rhizoma coptidis*

LI Peng-Yuan1,2, LI-Li2, LIANG Ri-xin2, WANG Lan2, LIU Yin2, MA Yin-Lian2, YU Ding-rong2, YAO Jia-qi 2, XIAO Yong-Qing2#   

  1. 1 School of Traditional Chinese Medicine,Capital Medical University, Beijing 100069;
    2 Institute of Chinese Mateia Madica,China Academy of Chinese Medical Sciences
  • Received:2014-11-28 Online:2015-03-28 Published:2015-03-28

Abstract: Objective To characterize the distribution of fingerprints of nine different compatibility formula (Formula 1~9) of Radix et Rhizoma rhei, Radix scutellariae and Rhizoma coptidis, three Chinese herbal medicines that comprise Sanhuang Xiexin decoction; to analysis the components of each formula, from which best effects of activating blood and resolving stasis were obtained. Methods High performance liquid chromatography (HPLC) was performed with Agilent C18 column, acetonitrile and 1% formic acid gradient elution, detection wavelength at 280 nm, column temperature of 30℃. Results 24 characteristic peaks were included in each fingerprint of compatibility formulae, and 9 peaks were identified with chemical reference. Formula 7, whose proportion of baicalin, anthraquinone glycosides, anthraquinones aglycone and berberine hydrochloride was 2.8∶1.7∶1.6∶1.0 approximately, was determined to be optimal to activating blood and resolving stasis. Conclusion The methods established of fingerprints and component analysis are stable and reliable to be used for quality control of component compatibility formulae. Furthermore, components analysis of Formula 7, the optimal formula for activating blood and resolving stasis, is expected to explore the correlation between compatibility and effect.

Key words: Sanhuang Xiexin decoction, activating blood and resolving stasis, fingerprint, component analysis

CLC Number: 

  • R284.1