主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (4): 236-240.doi: 10.3969/j.issn.1006-2157.2015.04.005

• Orignal Article • Previous Articles     Next Articles

Influence of different acupoints and intensity of stimulus on HPT axis in rats with syndrome of kidney-yang deficiency: a study based on orthogonal experimental design*

MIN You-jiang1,YAO Hai-hua1,ZHANG Hui2,DINGLI Li-qiang2,MIN Zhi-yun2,PEI Jia2 ,CHENG Li-hong1#   

  1. 1 Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Jiangxi 330006;
    2 Jiangxi University of Traditional Chinese Medicine
  • Received:2014-10-08 Online:2015-04-15 Published:2015-04-15

Abstract: Objective To study the operational parameters of acupuncture treatment for rats model of kidney-yang dificiency syndrome, with serum T3, TSH and hypothalamic TRH mRNA as indicators, and to find the main influencing factors and an optimal combination of factors so as to provide experimental evidence. Methods Rat model of kidney-yang deficiency syndrome was induced by intramuscular injection of hydrocortisone. L9 (34 ) orthogonal experiment was designed with four factors viz. acupoint, angle of twisting-rotation(angle), frequency of twisting-rotation (frequency), duration of twisting-rotation (duration) at three levels respectively. The rats were treated by acupuncture manipulation device controlled by computer. Serum T3 and TSH were measured by using ELISA, and the expression of hypothalamic TRH mRNA was determined by using RT-PCR. Results Frequency was the primary factor (P<0.01), duration was the secondary factor(P< 0.01 or P<0.05) and acupoint was the tertiary factor(P< 0.05 ). The optimal acupuncture factors were acupoint of Shenshu (BL23) , angle of 90°, frequency of 120 times/min and duration of 60 s or 120 s. Conclusion Frequency and duration were the essential factors of intensity of acupuncture, which were also the important factors to influence of acupuncture.

Key words: orthogonal experimental design, kidney-yang deficiency, T3, TSH, TRH mRNA, rats

CLC Number: 

  • R285.5