主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (5): 318-322.doi: 10.3969/j.issn.1006-2157.2015.05.007

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Protective effects of total saponrins in Panax Notoginseng on high glucose-induced injury in cultured rat retinal capillary endothelial cells(RRCECs)*

QIAO Yuan, DUAN Hui-hui, HUANG Jian-mei, TANG Min-ke#   

  1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102
  • Received:2015-03-04 Online:2015-05-15 Published:2015-05-15

Abstract: Objective To observe the effects of total saponins of Panax Notoginseng (PNS) protection and expression of intercellular adhesion molecules-1 (ICAM-1) in cultured rat retinal capillary endothelial cells (RRCECs) induced with high-concentration glucose. Methods Endothelial cells obtained from rat retinas were cultured in vitro, and the cells were identified using immunofluorescence. RRCECs were randomly divided into five groups: normal group, high glucose group, high glucose+20 mg/L PNS group, high glucose+50 mg/L PNS group, and high glucose+100 mg/L PNS group. Cell viability was measured with MTT method while cell number was detected with Trypan blue assay. The protein expression of ICAM-1 in RRCECs was measured by using Western blotting. Results According to MTT assay, the cell viability in high glucose+PNS (50 mg/L, 100 mg/L) groups were promoted after an action time of 48 hours and 72 hours (P<0.01). According to Trypan blue assay, after 48 hours and 72 hours, the cell number in high glucose+PNS (100 mg/L) group was increased (P<0.01); after 48 hours, the cell number in high glucose+PNS (50 mg/L) group was increased (P<0.05). After an action time of 72 hours, the expression of ICAM-1 in all high glucose+PNS groups were detected by using Western blotting. The result showed that the expression of ICAM-1 in RRCECs decreased with the rise of PNS concentration under high glucose condition (P<0.01). Conclusion PNS could protect RRCECs from damage caused by high glucose, and inhibit the expression of ICAM-1. Relation between protective effects of PNS and regulation of ICAM-1 expression in cultured RRCECs merits further study.

Key words: total Saponins of Panax Notoginseng, retinal capillary endothelial cells, intercellular adhesion molecules-1

CLC Number: 

  • R285.5