主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (5): 327-331.doi: 10.3969/j.issn.1006-2157.2015.05.009

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Effects of Danzhi Xiaoyao San on morphological changes of encephalic regions in the anxiety model rats*

MA Xiang-feng1, TANG Qi-sheng2#, ZHAO Rui-zhen2, DONG Ning1, WANG Rao1   

  1. 1 Beijing University of Chinese Medicine, Beijing 100029, China;
    2 Third Affliated Affiliated Hospital, Beijing University of Chinese Medicine
  • Received:2014-12-21 Online:2015-05-15 Published:2015-05-15

Abstract: Objective To observe the morphological changes in hippocampus and amygdaloid nucleus of rat model exposed to natural enemies and water drinking conflict, when administered with Danzhi Xiaoyao San (Cortex Moutan and Furctus Gardeniae Free Wanderer Powder) which has liver-soothing, heat-clearing and spleen-tonifying effects. Methods Evaluated by the open-field test, the male Wistar rats were randomly divided into four groups: normal group, model group, traditional Chinese herbal medicine group, and western medicine group, with 8 rats in each group. Rats in the model group, traditional Chinese herbal medicine group and western medicine group were exposed to natural enemies and water drinking conflict to establish anxiety model. From the 15th day on, rats in the normal group and model group were offered distilled water, and the rats in the western medicine group started on buspirone hydrochloride, while the rats in the traditional Chinese herbal medicine group were given Danzhi Xiaoyao San using intragastric administration. All the above intervention lasted for 7 consecutive days. Light microscope was used to observe the morphological changes in the hippocampus and amygdaloid nucleus. Results Somas of the neurons of the hippocampus and amygdaloid nucleus were relatively large and round in rats of normal group, and their brain cells lined up in an orderly concentrated manner. In the rats of model group, there was karyopyknosis of a large number of neurons, which were hyperchromatic and deformed; the cells were loosely arranged without patterns and there was increased space between the cells. Such feature was particularly prominent in the CA2 and CA3 regions of the hippocampus. Compared with the model group, cells in the traditional Chinese herbal medicine group and western medicine group appeared significantly better and the cell layout was relatively in order. Conclusion The Chinese herbal medicine, Danzhi Xiaoyao San, the key liver-soothing, heat-clearing and spleen-tonifying formula, may repair the encephalic region injury related to the rat’s Papez circuit and also facilitate the recovery.

Key words: exposure to natural enemies and water drinking conflict, liver-soothing, heat-clearing and spleen-tonifying method, morphological changes, Danzhi Xiaoyao San, rats

CLC Number: 

  • R285.5