主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (6): 377-382.doi: 10.3969/j.issn.1006-2157.2015.06.004

Previous Articles     Next Articles

Effects of heat-clearing and blood-activating combination on acute cerebral ischemia of heat toxin pattern in rats via NF-κB signaling pathway*

LIANG Xiao1, ZHANG Yun-ling2#, WANG Xin-xiang2, LIU Xue-mei2, ZHENG Hong2, YAO Ting1, WANG Jian-wei1   

  1. 1 Beijing University of Chinese Medicine,Beijing 100029;
    2 Dongfang Hospital, Beijing University of Chinese Medicine
  • Online:2015-06-15 Published:2015-06-15

Abstract: Objectives To study the synergetic effects of heat-clearing and blood-activating combination in the treatment of acute cerebral ischemia of heat toxin pattern. Methods Animal model of acute cerebral ischemia of heat toxin pattern was established by using middle cerebral artery occlusion (MCAO) and carrageenan injection in rats. Rats were randomly divided into eight groups: normal group, sham group, ischemia 1.5 h/reperfusion 72 h group, Kudiezi(KDZ) injection group (heat-clearing, group A), Xueshuantong(XST) injection group (blood activating, group B), KDZ1.8 mL/kg+XST20 mg/kg (group C), KDZ1.8 mL/kg+XST80 mg/kg (group D), and KDZ7.2 mL/kg+XST20 mg/kg (group E). Infarction area ratios was measured by TTC staining; activity or content of NF-κB p65 and IKKβ of cerebral tissues, by Western bolt; gene expression of NF-κB p65 and IKKβ, by real- time PCR. Results Cerebral tissue ischemia in all treatment groups was reduced (P<0.05). Heat-clearing and blood-activating combined treatment improved significantly (P<0.01) compared with the model group, yet there were no significant differences between these groups. NF-κB p65 and IKKβ in model group were highly expressed (P<0.01) compared with the normal group. They both decreased in all treatment groups. NF-κB p65 protein expression in group C and E significantly decreased compared with the model group (P<0.01); and they also decreased (P<0.05) in group A and B. Expression of IKKβ protein decreased in combination treatment groups (group C, D, E and A) markedly (P<0.01). In addition, IKKβ protein expression in groups C, D and E were lower than that in group B significantly (P<0.01); It was markedly lower in groups C and E than in KDZ group (P<0.01). Expression of NF-κBp65、IKKβ mRNA in the model group was higher (0.48%) than that in the normal group (0.37%). NF-κB p65 and IKKβ mRNA expression in each treatment group decreased compared with the model group. Expression of NF-κB p65 and IKKβ mRNA in group D and the expression of IKKβ mRNA in group E was lower than the model group; the expression of NF-κB p65 mRNA in group E was significantly different compared with the model group. Conclusion Heat-clearing and blood-activating combination has synergetic effects on acute cerebral ischemia via regulating the protein and gene expression of NF-κB p65 and IKKβ in NF-κB signaling pathway.

Key words: acute cerebral ischemia, heat toxin pattern, inflammatory reaction, NF-κB signaling pathway, rats

CLC Number: 

  • R285.5