主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (6): 383-387.doi: 10.3969/j.issn.1006-2157.2015.06.005

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Effects of liver-soothing heat-clearing and spleen-fortifying method on the expressions of cerebral bax and bcl-2 in rats with generalized anxiety disease*

DONG Ning1, TANG Qi-sheng2#, ZHAO Rui-zhen2, LUO Bin2, WANG Wen-long1   

  1. 1 Beijing University of Chinese Medicine, Beijing 100029;
    2 The Third Affiliated Hospital, Beijing University of Chinese Medicine
  • Received:2014-10-20 Online:2015-06-15 Published:2015-06-15

Abstract: Objective To explore the effects of liver-soothing heat-clearing and spleen-fortifying method on the expressions of bax and bcl-2 in cerebral Papez’s circuit in rat with generalized Anxiety Disease (GAD). Methods A total of 40 male Wistar rats were divided into normal group, model group, treatment group, and control group, with 10 rats in each group. GAD model was established by using the stimulation of uncertain empty drinking bottle. Then the treatment group and control group were intragastrically given Danzhi Xiaoyao San (Cortex Moutan and Fructus Gardeniae Free Wanderer Powder) and Buspirone Hydrochloride once a day for 7 consecutive days respectively. The model group was given distilled water once a day for a week. The expressions of bax and bcl-2 in rat hippocampus and mamillary body were measured by applying western blot assay after resecting rat brains. Results The expression of bax in hippocampus and mamillary body of model group increased significantly compared with normal group (P<0.05 or P<0.01) and the expression of bcl-2 decreased markedly (P<0.01). Compared with model group, the level of bax in hippocampus and mamillary body of treatment group and control group was reduced (P<0.05 or P<0.01) and the level of bcl-2 increased significantly (P<0.05 or P<0.01). Conclusion The therapeutic method of liver-soothing heat-clearing and spleen-fortifying method represented by Danzhi Xiaoyao San could reduce the level of bax in hippocampus and mamillary body of GAD rat brain, elevate the level of bcl-2 and reduce bax/bcl-2, prevent and inhibit cell apoptosis in Papez’s circuit and then ameliorate anxiety symptoms.

Key words: liver-soothing heat-clearing and spleen-fortifying method, generalized anxiety disease, Papez’s circuit, bcl-2, bax, rats

CLC Number: 

  • R285.5