主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (6): 393-399.doi: 10.3969/j.issn.1006-2157.2015.06.007

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Quality representation and correlation analysis of Puhuang based on quality evaluation model of drug system*

LI Gang1, XU Shu-ya1, ZHAO Li-min1, LU Guang-ying, SUN Dao-han1, WANG Ya-li1, Tang Xue-yang1, PENG Ping1, JIANG Yan-yan1,2, SHI Ren-bing1,2#   

  1. 1 Key Unit of Exploring Effective Substances of Classical and Famous Formulas of State Administration of Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102;
    2 Quality Control Technology and Engineering Center of Chinese Medicine, Beijing Municipal Commission of Education
  • Received:2014-11-20 Online:2015-06-15 Published:2015-06-15

Abstract: Objective To establish quality representation and correlation analysis method of Puhuang (Pollen Typhae Angustifoliae) pieces based on drug system and evaluate Puhuang quality comprehensively. Methods HPLC was used for simultaneous determination of five major active ingredients and their total amount in 16 batches of Puhuang (Pollen Typhae Angustifoliae.) decocting pieces, including typhaneoside, isorhamnetin-3-O-neohesperidoside, isorhamnetin, stigmasterol, and β-sitosterol. Ferric chloride-potassium ferricyanide colorimetric method was used for measuring the content of phenols in flavonoids while vanillin-perchloric acid colorimetric method was used for the determination of pollen by measuring the total sterol content of pollen Typhae. Non-correlation coefficient, non-correlation degree, and correlation degree were used to represent quality correlation between samples and reference products. Results Levels of active ingredients were higher in No.7, 3, 5, 4, 10 and 13 Puhuang pieces than No.8 (reference product). No.5, 7, 6, 10, 1, and 13 batches had high relevance with the reference product No.8. Consolidated quality characterization and correlation analysis showed that the quality of No. 7, 5, 10, and 13 were superior. Conclusion Based on drug quality evaluation system, measurement of active ingredient levels, total amount and relative ratio combined with correlation analysis of reference product with definite efficacy could offer a comprehensive assessment of quality of Puhuang decocting pieces. This method could provide evidence for screening, quality control and application of Puhuang decocting pieces. It also serves as references for quality evaluation of Chinese materia medica both in methodology and application.

Key words: Puhuang (Pollen Typhae Angustifoliae), quality evaluation, correlation analysis, Typhaneoside, Isorhamnetin-3-O-neohesperidoside, Isorhamnetin, Stigmasterol, β-sitosterol

CLC Number: 

  • R284.1