主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (6): 405-410.doi: 10.3969/j.issn.1006-2157.2015.06.009

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Spectrum-effect relation in antioxidant activity of Menthae haplocalycis Herba based on DPPH and FRAP assay*

XU Jing-jing, LIU Bin#   

  1. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 100029
  • Received:2014-12-15 Online:2015-06-15 Published:2015-06-15

Abstract: Objective To explore the active ingredients of Menthae Haplocalycis Herba by investigating the correlation between HPLC spectrum and antioxidant activity. Methods We established DPPH[1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl,DPPH] and FRAP(ferric ion reducing antioxidant power, FRAP) assay to determine the antioxidant activity of Menthae Haplocalycis Herba. The spectrum-effect correlation was studied by using PLS(Partial least squares). HPLC-MS/MSn was applied to identify their structures. Results Six compounds including hesperidin, diosmin, buddleoside, thymusin and xanthomicrol, which made great contributions to the DPPH scavenging activity, were screened; eight compounds, including hesperidin, buddleoside, rosmarinic acid and trans Salvianolic acid J, were obtained which made great contributions to the total antioxidant activity. Conclusion It is an effective way to characterize spectrum-efficacy correlation and clarify the active components of Chinese medicinal.

Key words: Menthae Haplocalycis Herba, antioxidant activity, spectrum-effect relationship, DPPH, FRAP, PLS

CLC Number: 

  • R284.1