主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (7): 462-466.doi: 10.3969/j.issn.1006-2157.2015.07.008

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Mechanism of invigorating kidney and activating blood recipe for the treatment of diabetic erectile dysfunction in rats

YUE Zeng-bao, MA Wen-jun, QIN Mao, LIU Bao-xing, KE Ming-hui, WANG Chuan-hang#   

  1. China-Japan Friendship Hospital, Beijing 100029
  • Received:2015-01-04 Online:2015-07-30 Published:2015-07-30

Abstract: Objective To study the therapeutic effect and mechanism of invigorating kidney and activating blood(IKAB)recipe, a Chinese herb compound, on diabetic erectile dysfunction (DED) in rats. Methods 40 male SD rats were randomly divided into four groups (each n=10):normal group, model group, low-dose IKAB group (low-dose group)and high-dose IKAB group(high-dose group). The rat model with 2 type of diabet mellitus was induced by intraperitoneal injection of streptozotocin (STZ). Normal group and model group were intragastrically administered (ig.) with normal saline, low-dose group was ig. 3 g/kg of IKAB and high-dose group was ig. 6 g/kg IKAB for consecutive 8 weeks. The penis viscera index was measured;the intracavernous pressure (ICP)were detected by Biopack electrophysiological instrument; the expressions of pAKT and AKT were detectd by Western blotting; cell apoptosis of corpus cavernosum smooth muscle were measured by using TUNEL method and the pathological changes in penile tissue were observed by using Masson trichrome staining. Results Compared with model group, the penis viscera index of both IKAB groups had no statistical differences (P>0.05); the expressions of pAKT and AKT increased significantly(P<0.05); although the ratio of cell apoptosis of corpus cavernosum smooth muscle decreased, the statistical differences were not shown (P>0.05).Furthermore, ICP and ICP/MAP increased markedly in high-dose group and the collagen fiber proportion decreased in low-dose group compared with model group (P<0.05).Conclusion The mechanism of invigorating kidney and activating blood recipe increasing ICP in rat model with DM is related to increasing the expression of pAKT, inhibiting cell apoptosis of corpus cavernosum smooth muscle and reducing collagen fibers formation in penis.

Key words: diabetes millitus, erectile dysfunction, invigorating kidney and activating blood, pAKT/AKT pathway, cell apoptosis, rats

CLC Number: 

  • R285.5