主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (7): 467-472.doi: 10.3969/j.issn.1006-2157.2015.07.009

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Mesaconitine extracted from Radix Aconiti Lateralis Preparata Aconite inhibited proliferation and induced apoptosis of human erythroleukemia (K562) cell line

GUAN Xin, YANG Tong-hua#, ZHANG Ai-ling, SU Mian-cheng, ZHAO Ren-bin, ZHANG Chao-ran, HE Li-li   

  1. Department of Hematology, First People's Hospital of Yunnan Province, Yunnan 650032
  • Received:2015-01-13 Online:2015-07-30 Published:2015-07-30

Abstract: Objective To study the biological effect of mesaconitine extracted from Fuzi (Radix Aconiti Lateralis Preparata Aconite) on human erythroleukemia (K562) cells lines. Methods The contents of mesaconitine and benzoylmesaconine were determined by using HPLC. After K562 cells were treated with mesaconitine standard substance, the leukemia cell morphology was observed under an electron microscope microscope. And the cell cycle, apoptosis of leukemic cells were measured by flow cytometry. Results Mesaconitine of 25~50 mg/L significantly inhibited K562 cells proliferation in dose- and time- dependent manner(P<0.05). Apoptosis was induced by mesaconitine at the dose of 25~50 mg/L(P<0.05). The ratio of cells at S phase increased and that of cells at G1 phase decreased(P<0.05). Conclusion Mesaconitine extracted from Fuzi inhibited proliferation and induced apoptosis of K562 cell line.

Key words: mesaconitine, K562 cells, cell proliferation, cell cycle, apoptosis

CLC Number: 

  • R285.5