主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (7): 473-480.doi: 10.3969/j.issn.1006-2157.2015.07.010

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Quality representation and correlation analysis of Fructus Gardenia based on characteristic spectrum of medicinal system

XU Shu-ya1, LI Gang1, MEI Ying-ying1, LU Guang-ying1, ZHAO Li-min1, TANG Xue-yang1, ZHANG Lu1, LI Huan-juan1, PENG Ping1, SHI Ren-bing1, 2 #   

  1. 1 Key Unit of Exploring Effective Substances of Classical and Famous Formulas of State Administration of Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102;
    2 Quality Control Technology and Engineering Center of Chinese Medicine, Beijing Municipal Commission of Education
  • Received:2014-12-30 Online:2015-07-30 Published:2015-07-30

Abstract: Objective To establish the quality representation and correlation analysis method based on the characteristic spectrum of medicinal system of raw decoction pieces of Zhizi (Fructus Gardenia), so as to evaluate quality of Zhizi pieces effectively and accurately. Methods Using established HPLC-PDA method to characterize the quality of Zhizi characteristic chromatogram of medicinal system. The quality of 16 batches of Zhizi pieces was represented according to the number and chemical type of characteristic peaks on chromatogram, and the quantity of 16 batches of Zhizi pieces was represented based on the content of characteristic index components of gardenoside, genipin 1-gentiobioside, chlorogenic acid, iridoids (represented by gardenoside) and phenylpropionic acids (represented by chlorogenic acid). The content of total terpenoids was determined by using vanillin-perchloric acid colorimetric method and the content of total phenols was detected by using chloride-potassium ferricyanide colorimetric method. Correlation analysis of quality and quantity representations was then performed referring to the benchmark pieces. Results As the reference substance, characteristic spectrum of Lot.6 contains nine characteristic peaks including four peaks of iridoids and five peaks of phenylpropionic acids, which all appeard on the chromatograms of 16 batches Zhizi pieces. Lot.3, 4, 7, 8, 13 Zhizi pieces had more effective index components than that of Lot.6, and Lot. 7, 1, 9, 4, 3, 12, 5, 14 pieces had higher correlation degree to Lot.6. After comprehensive evaluation, the quality of Lot. 3, 4, 6, 7 were better. Conclusion The established HPLC methods by quality and quantity representation of characteristic spectrum of 16 batches of Zhizi pieces is simple and accurate. And the evaluation mode of quantity and quality and their correlation based on Zhizi medicinal system, concerning on systemic correlation and application validity, can evaluate the quality of Zhizi pieces effectively and accurately.

Key words: Zhizi(Fructus Gardenia), characteristic chromatogram, quality evaluation, correlation analysis, gardenoside, genipin 1-gentiobioside, chlorogenic acid

CLC Number: 

  • R284.1