主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (7): 490-495.doi: 10.3969/j.issn.1006-2157.2015.07.013

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Effects of acupuncture and moxibustion on serum granulocyte colony stimulating factors in CTX-induced tumor-bearing mice

LU Mei1 , FU Han-lei1, CAO Da-ming1#, ZHAO Xi-xin1, LI Jian-wei2, WANG Guang-an1, ZHANG Li1   

  1. 1 Henan University of Chinese Medicine, Henan 450008;
    2 First Affiliated Hospital of Henan University of Chinese Medicine
  • Received:2015-01-09 Online:2015-07-30 Published:2015-07-30

Abstract: Objective To study the content of serum granulocyte macrophage colony stimulating factor(GM-CSF)and granulocyte colony stimulating factor(G-CSF)in cyclophosphamide(CTX)-induced tumor-bearing mice before and after acupuncture and moxibustion treatment, so as to find out the mechanism of acupuncture and moxibustion treatment on recovery from myelosuppression and increasing of leukocyte counts. Methods 64 Kunming male mice of clean grade were transplanted subcutaneously S180 sarcoma cells to induce the transplanting tumor model, then were randomly divided into control group, model group, acupuncture group and moxibustion group (each n=16) according to the body weight. Mice in model group, acupuncture group and moxibustion group were intraperitoneally injected with CTX to establish CTX-induced tumor-bearing mice model, and the control group were injected intraperitoneally with normal saline. Acupoints Dazhui(GV14), Geshu (BL17), Shenshu (BL23) and Zusanli (ST36) were needled in acupuncture group and were moxa moxibusted in moxibustion group once daily at regular time for consecutive 5 d. Peripheral leukocyte counts were measured by detecting the blood collected from caudal vein before modeling and at every day of treatment procedure. The serum concentrations of GM-CSF and G-CSF were determined by using ELISA method at the third day of treatment and one day after five-day-treatment. Results Compared with control group, peripheral leukocyte counts decreased, even to the lowest level at the fourth day in the three groups of CTX-induced tumor-bearing mice, and the serum concentrations of GM-CSF and G-CSF also decreased in the three groups significantly(P<0.05). At the fifth day of treatment, leukocyte counts started to increase, and the serum concentrations of GM-CSF and G-CSF increased significantly compared with model group(P<0.05). At the day after treatment, leukocyte counts increased to nearly normal level and the serum concentrations of GM-CSF and G-CSF increased dramatically compared with model group. Conclusion CTX damaged the myeloid element to reduce peripheral leukocyte counts and the serum concentrations of GM-CSF and G-CSF. Acupuncture and moxibustion can obviously increase the concentration of GM-CSF and G-CSF, that is to say, both the Chinese medical treatment can induce colony growth of the granulocyte precursor and macrophage precursor cells and stimulate differentiation of the hematopoietic cells, leading to proliferation, differentiation and maturation of the myeloid hematopoietic cells, resulting in recovery from myelosuppression and increasing of leukocyte counts induced by chemotherapy.

Key words: myelosuppression, acupuncture, moxibustion, leukocyte counts, CSF, cyclophosphamide, mice

CLC Number: 

  • R245