主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (8): 519-523.doi: 10.3969/j.issn.1006-2157.2015.08.003

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Regulatory effects of spleen-yin nourishing formula on endoplasmic reticulum stress in spleen-yin deficiency diabetic rats*

LIANG Li-na1, ZHAN Li-bin2#, HU Shou-yu2, SUI Hua1, ZHENG Lu-ping1, LI Zhao1, ZHANG Fu-liang3   

  1. 1 College of Integrative Medicine and Institute of Integrative Medicine, Dalian Medical University, Liaoning 116044;
    2 The Second Affiliated Hospital of Dalian Medical University;
    3 Department of Public Health, Dalian Medical University
  • Received:2014-08-28 Online:2015-08-30 Published:2015-08-30

Abstract: Objective To investigate the effects of spleen-yin nourishing formula, ZiBu PiYin Recipe (ZBPYR), on endoplasmic reticulum stress (ERS) in the hypothalamus of spleen yin deficiency diabetic rats. Methods Rats were randomly divided into five groups: control (cont) group, Diabetes Mellitus (DM) group, spleen yin deficiency (pi) group, piDM group, and ZBPYR group. The expressions of p-PERK, p-eIF2α, eIF2α and GRP78, which were endoplasmic reticulum stress markers in the hypothalamus of rats, were measured using western blotting Methods. Results The protein expressions of p-PERK, p-eIF2α and GRP78 in the hypothalamus of DM group and piDM group were elevated, as compared with cont group (P<0.05). Their levles were decreased in the ZBPYR group, compared with DM group and piDM group (P<0.05). In addition, no significant difference in eIF2α expression was detected among different groups. Compared with the cont group, GRP78 mRNA expression in hypothalamus increased in the DM group, pi group and piDM group (P<0.05). The expression of GRP78 mRNA was lower in ZBPYR group than in the DM and piDM groups (P<0.05). Conclusion Endoplasmic reticulum stress seems to be present in the hypothalamus in the DM and piDM groups. Formulas that enrich and tonify the spleen yin have the effects of alleviating endoplasmic reticulum stress.

Key words: spleen yin deficiency diabetes, cognitive decline, hypothalamus, endoplasmic reticulum stress, ZiBu PiYin Recipe, rats

CLC Number: 

  • R285.5