主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (8): 532-534.doi: 10.3969/j.issn.1006-2157.2015.08.006

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Effects of Zengye Decoction on the expression of AQP3 and VIP in colon tissues of mice with slow transit constipation*

MA Li-qian, LI Cheng-yin, WANG Yue#   

  1. No.1 School of Clinical Medicine, Nanjing University of Chinese Medicine, Jiangsu 210046
  • Received:2015-03-10 Online:2015-08-30 Published:2015-08-30

Abstract: Objective To investigate the effects of Zengye Tang (Fluid-increasing Decoction) on the expression of Aquaporin-3 (AQP3) and Vasoactine Intrestinal Peptide (VIP) in colon tissues of mice with Slow Transit Constipation (STC) and to explore the correlation between AQP3 and VIP. Methods 60 ICR mice were randomly divided into 4 groups, namely control group, model group, mosapride group and Zengye Decoction group. Each group except control group was given diphenoxylate to establish the STC model. Control group was given saline. After the establishment of the model, Zengye Decoction group was given the Zengye Decoction while mosapride group was given mosapride. Control group and model group were given saline. The expression of AQP3 and VIP in colon tissues in each group was measured by using immunohistochemistry staining. Mean density (MD) was used to analyze the correlation between AQP3 and VIP. Results Mean density of AQP3 and VIP in colon tissues in model group were markedly lower than that in control group (P<0.05). Levels of AQP3 and VIP in mosapride group and Zengye Decoction group were significantly higher than that in model group (P<0.05). AQP3 and VIP in Zengye Decoction group were not statistically different from that in mosapride group (P>0.05). The levels of AQP3 and VIP in each group were positively correlated. Conclusions Zengye Decoction can increase the expression of AQP3 and VIP in colon tissues of STC mice, which may explains the mechanism of its efficacy. The expressions of AQP3 and VIP in colon tissues were correlated, probably due to a shared signal pathway.

Key words: Zengye Decoction, slow transit constipation, Aquaporin-3, vasoactine intrestinal peptide, immunohistochemistry, mice

CLC Number: 

  • R285.5