主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (8): 546-550.doi: 10.3969/j.issn.1006-2157.2015.08.009

Previous Articles     Next Articles

Rapid analysis of chemical compositions in the flavonoid fraction of Mentha haplocalyx Briq. with LCMS-IT-TOF*

CHEN Xiang-yang1, ZHANG Le1, WU Ying2, WANG Wei-hua3, LIU Bin1#   

  1. 1 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102;
    2 School of Preclinical Medicine, Beijing University of Chinese Medicine;
    3 National Institute of Metrology
  • Received:2015-02-08 Online:2015-08-30 Published:2015-08-30

Abstract: Objective To rapidly analyze chemical compositions in the flavonoid fraction of Mentha haplocalyx Briq. Methods The liquid chromatography-mass spectrometry-ion trap-time-of-flight (LCMS-IT-TOF) method was applied to analyze the flavonoid fraction of Mentha haplocalyx Briq.The chromatographic separation was performed on Sunfire C18 column(4.6 mm×150 mm,5 μm)with gradient elution of methanol (A)-water (B). Multistage mass spectrometry data of different compounds was obtained by using electrospray ionization source and monitoring in positive and negative ion modes. Results Twelve flavonoids, including four flavonoid glycosides, six flavonoid aglycones, a flavonol glycoside and a flavanone glycoside, were identified based on the characteristic MS/MS fragment ions data with chemical standards and published literatures. These constituents are mainly hesperidin, rutin, apigenin-7-O-rutinoside, diosmin, luteolin-7-O-rutinoside, linarin, 5,6-dihydroxy-7,8,3′,4′-tetramethoxy-flavone, gardenin B, 5,6-dihydroxy-7,8, 4′-trimethoxy flavone, acacetin, 5-hydroxy-6,7,8,3′,4′-pentamethoxy flavone and diosmetin. Conclusion LCMS-IT-TOF is a rapid and effective technology to obtain the chemical compositions in the flavonoid fraction of Mentha haplocalyx Briq., which is of great significance for further study.

Key words: Mentha haplocalyx Briq., flavonoid fraction, liquid chromatography-mass spectrometry-ion trap-time-of-flight

CLC Number: 

  • R284.1