主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (9): 611-618.doi: 10.3969/j.issn.1006-2157.2015.09.007

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Effects of TCM compound of resolving stagnation for tranquilization on neurotransmitters and brain-derived neurotrophic factors in depression rat model by chronic unpredictable stress*

ZOU Haiyan1,2, YANG Yilong1,2, TU Hengjing3, YAO Xiaoquan1,2, WANG Yali1,2, LIU Sisi1,2, OUYANG Junyao1,2, WANG Haizheng1,2, ZHAO Hui1,2, WANG Lei1,2, ZHANG Chi3, ZHANG Qiuxia1,2#   

  1. 1 School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069; 2 Beijing Key Laboratory of TCM Collateral Disease Theory Research;3 Beijing University of Chinese Medicine
  • Received:2014-11-17 Online:2015-09-30 Published:2015-09-30

Abstract: Objective To observe the effect of TCM compound of resolving stagnation for tranquilization (RST) on neurotransmitters and brain derived neurotrophic factors (BNDF) in depression rat model induced by chronic unpredictable stress (CUS), and to reveal the mechanism of RST on the treatment of depression. Methods Altogether 60 male SD rats were randomly divided into normal group, model group, high-, mid- and low- dose RST groups at dosage of 8.2, 16.3, 32.7 g/kg, and fluoxetine group with dose of 10 mg/kg. The rats of four therapy groups were administered intragastrically drugs with undergoing CUS for consecutive thirty-five days. The behavioral changes of rats were observed by open field test and novelty-suppressed feeding-test. The content of dopamine (DA), 5-hydroxeytryptamine(5-HT), 5-hydroxyindole acetic acid (5-HIAA) and norepi-nephrine(NE) in hippocampus and cortex were determined by HPLC. And the expression of BDNF in hippocampus was measured by Western blot. Results Compared with the normal group, the rats’ weight increased slowlier and the spontaneous activity decreased in model group; the contents of 5-HT and NE in cortex and the content of 5-HT, 5-HIAA and NE in hippocampus significantly reduced (P<0.05). Compared with the model group, the spontaneous activity significantly increased in all therapy groups; the contents of 5-HT and NE in cortex dramatically increased in fluoxetine group and mid-, high- dose group (P<0.05); the contents of 5-HT and 5-HIAA in hippocampus significantly increased in all three RST groups (P<0.05); the content of NE in hippocampus significantly increased in low- and high- dose group (P<0.05). The expression of BDNF in hippocampus significantly increased in fluoxetine group and high-dose RST group (P<0.05). Conclusion The anti-depression mechanism of TCM compound formulated by principle of resolving stagnation for tranquilization may be related to the effects of increasing neurotransmitters and up-regulation of BDNF.

Key words: relieving stagnation and tranquilizing, chronic unpredictable stress(CUS), neurotransmitters, brain derived neurotrophic factor(BDNF) , rats

CLC Number: 

  • R285.5