主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (9): 606-610.doi: 10.3969/j.issn.1006-2157.2015.09.008

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Effects of serum containing modified Shengmai liquid on migration and adhesion of non small cell lung cancer cells*

CHEN Hui, JIANG Miao, XIN Lili,QU Jinglian, XU Muge,SU Yinxu,GONG Jiening#   

  1. Nanjing University of Chinese Medicine, Jiangsu 210046
  • Received:2015-03-02 Online:2015-09-30 Published:2015-09-30

Abstract: Objective To study the effects and mechanism of serum containing modified Shengmai liquid (MSL)on migration and adhesion of non small cell lung cancer (NSCLC) cells. Methods Rabbit serum containing MSL was prepared by intragastric administration (i.g.)with MSL at the dose of 18 g/kg and control serum was prepared by i.g. normal saline. NSCLC cells were cultured, and those in logarithmic growth phase collected were divided into four groups: 10% control serum(CS) group, 2.5%, 5%, 10% MSL serum group. Migration cells were detected by using Transwell invasion assay, and adhension rate was measured by using matrigel adhensive assay. The expressions of N-cad and vimentin were determined by RT-PCR. Results The number of migration cells decreased as the concentration of serum containg MSL increased:migration rate of 5% MSL group was 25.64% and that of 10% MSL group was 13.31%(P<0.01). As the concentration of serum containing MSL increased and the duration of treatment prolonged, heterogeneous adhesion rate decreased and was negatively correlated with serum concentration and duration; while the gene expressions of N-cad and vimentin decreased in dose- and time- dependent manner. Conclusion The mechanism of inhabitation migration and reduction heterogeneity adhension of MSL on NSCLC cells would be related to the epithelial-mesenchymal transition.

Key words: serum containing modified Shengmai liquid, non small cell lung cancer, migration, adhesion, cell cultured

CLC Number: 

  • R285.5