主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (9): 619-623.doi: 10.3969/j.issn.1006-2157.2015.09.009

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Gene and protein expression of B cell-activating factor and its receptor in MRL/lpr lupus mice with different constitutions*

WANG Dongjian1,2, SU Li1#,WANG Xiao1, QU Huanru1,MAO Jianchun1,GU Junhua1,DING Zhijiang, ZHOU Jun1   

  1. 1 Longhua Hospital, Shanghai University of TCM, Shanghai 200032; 2 Shanghai Eighth People Hospital
  • Received:2015-03-20 Online:2015-09-30 Published:2015-09-30

Abstract: Objective To establish the method to distinguish the constitution of MRL/lpr lupus mice and to study the gene and proteinexpression of renal B cell-activating factor (BAFF), B cell stimulating factor receptor (BAFF-R), so as to reveal the factors at molecular biological level leading to the differences of constitution. Methods Firstly, MRL/lpr female mice of 12-week age were classified into cold constitution, heat constitution and normal constitution. Secondly, 20 mice were randomly selected respectively from cold constitution group, heat constitution group and normal constitution group. Thirdly, every 10 mice in each group were sacrificed at 12-week age and at 20-week age respectively. Then, expressions of renal BAFF mRNA, BAFF-R mRNA, BAFF protein and BAFF-R protein were examined by real-time PCR and Western Blot. Results Expressions of BAFF mRNA ,BAFF protein and BAFF-R protein were the lowest in the cold constitution group of all the groups. The expression of BAFF-R mRNA showed no significant difference among all groups. Conclusion The constitutional differences could be related to the expressions of renal BAFF mRNA ,BAFF protein and BAFF-R protein .

Key words: systemic lupus erythematosus, constitution, B cell-activating factor, B cell-activating factor receptor, mice

CLC Number: 

  • R285.5