主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (9): 624-628.doi: 10.3969/j.issn.1006-2157.2015.09.010

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Characteristic of intestinal absorption of euphorbia factor L1 by rats single pass intestinal perfusion moder in situ*

ZHANG Xiuting1, WANG Yingzi1#, LI Shaojing2,DUAN Feipeng1, WANG Qing1, ZHANG Chunni1, LI Fengying1,LI Wenhua1, LUO Shengxiu1   

  1. 1 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102; 2 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences
  • Received:2015-03-18 Online:2015-09-30 Published:2015-09-30

Abstract: Objective To study the characteristic of absorption of euphorbia factor L1 in intestine of rats, and to observe the effects of P-glycoprotein(P-gp)and multidrug resistance-associated protein(MRP2)on intestinal absorption of euphorbia factor L1. Methods The contents of euphorbia factor L1 of intestinal perfusion fluid of duodenum, jejunum, ileum and colon in the rats in situ single-pass intestinal perfusion model were determined by using HPLC. The drug absorption rate constant(Ka) and the apparent absorption coefficient(Papp) in four intestinal regions were calculated. Results Ka and Papp of euphorbia factor L1 at colon were the highest of the whole rat intestine. Significant increase of Ka and Papp showed at rat colon when co-perfused with verapamil hydrochloride, by contrast, decrease of Ka and Papp found when co-perfused with indomethacin. Conclusion We inferred that verapamil hydrochloride be the substrate of P-gp and that indomethacin be not the substrate of MRP2.

Key words: single-pass intestinal perfusion, euphorbia factor L1, P-glycoprotein, multidrug resistance-associated protein, rats

CLC Number: 

  • R969.1