主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (10): 686-689.doi: 10.3969/j.issn.1006-2157.2015.10.009

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Effects of Yiqi Huoxue Jiedu Fang on gene expression of cytokines involving NF-κB signal pathway in collagen II-induced arthritic rats*

DONG Bin, LOU Lixia, WEI Yi, ZHAO Jiuli, JIN Qiushuo, CHAI Limin#   

  1. Key Laboratory of Chinese Internal Medicine, Ministry of Education &
    Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700
  • Received:2014-12-19 Online:2015-10-30 Published:2015-10-30

Abstract: Objective To study the gene expressions of key cytokines involving NF-κB signaling pathway in collagen II-induced arthritic (CIA) rats treated with Yiqi Huoxue Jiedu Fang (qi-tonifying blood-activating and toxin-removing formula). Methods We used collagen II-induced arthritic (CIA) rats by bovine II collagen as an animal model and Leflunomide as controls. CIA rats received treatment with Yiqi Huoxue Jiedu Fang. The levels of inflammatory cytokines in serum were measured with protein chip assay, and hematoxylin dyeing was used to observe the histopathological changes. The gene expressions of the key cytokines in NF-κB signal pathway were detected by real-time PCR. Results This formula inhibited the proliferation of synovial tissues, reduced inflammatory exudation and narrowed the range of fibroid necrosis. It also down-regulated inflammatory cytokines (TNF, IL-1, IL-5, IL-6 and IL-10) in serum of CIA rats. The gene expressions of NF-κB and IKK decreased in CIA rats treated with this formula. Conclusion Yiqi Huoxue Jiedu Fang could regulate the expressions of inflammatory cytokines and the key factor of NF-κB signal pathway for inhibiting inflammatory reaction in injured tissues, restoring the joint injury. Those could be the key active mechanism of RA treated with this formula.

Key words: Yiqi Huoxue Jiedu Fang, collagen II-induced arthritis, inflammatory cytokines, NF-κB signal pathway, rats

CLC Number: 

  • R285.5