主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (11): 740-744.doi: 10.3969/j.issn.1006-2157.2015.11.005

• Original Article • Previous Articles     Next Articles

Effects of Naoluo Xintong decoction on mRNA expressions of 5-hydroxytryptamine, dopamine, norepinephrine and 5-hydroxyindoleacetic acid in corpus striatum tissues of MCAO rats*

HE Ling, WEN Anhui, YAN Zhengyuan, HU Jianpeng, XU Wei, TAN Hui, WANG Jian#   

  1. Key Laboratory of Xin′an Medicine under Ministry of Education and Anhui Province ,Anhui University of Traditional Chinese Medicine, Anhui 230038
  • Received:2015-03-10 Online:2015-11-30 Published:2015-11-30

Abstract: Objective To study the mechanism of protective effect of Naoluo Xintong decoction(NLXTD), a traditional Chinese medicine compound, on cerebral ischemia-reperfusion injury in rats. Methods Altogether 100 male SD rats were randomly divided five groups( each n=20 ): sham-operated group, model group, premedication group, three-day-medication group and seven-day-medication group. After modeling, three-day-medication group was administered intragastrically (i.g.) NLXTD for consecutive three days, and the seven-day-medication group for seven days at the dose of 8.54 g/kg daily twice. The premedication group was treated with NLXTD of 8.54 g/kg daily twice for consecutive seven days before modeling .The model group and sham-operated group were treated with the distill water for seven days. Regional cerebral blood flow (rCBF) of corpus striatum of the side of cerebral infarction was measured by using laser Doppler imager (LDF). Then all the rats were sacrificed and the corpus striatum were dissected out from brain. Infarct volume was determined by using 2,3,5-triphenyltetrazolium chloride (TTC) staining, and the mRNA expressions of 5-hydroxytryptamine(5-HT), dopamine(DA), norepinephrine(NE) and 5-hydroxyindoleacetic acid(5-HIAA) in the corpus striatums were detected by qRT-PCR. Results Compared with the sham-operated group, the rats of the model group, with obvious cerebral ischemia, suffered more cerebral infarction volume(P<0.01),and the levels of mRNA expression of 5-HT,DA,NE and 5-HIAA in corpus striatum significantly decreased(P<0.01). Compared with the model group, the cerebral infarction volume of premedication group, three- and seven- day-medication group dramatically decreased by increasing the cerebral blood flow(P<0.01); the mRNA expressions of 5-HT, DA, NE and 5-HIAA increased significantly(P<0.01). Conclusion NLXTD could prevent and cure cerebral injury of MCAO rats by increasing the cerebral blood flow and decreasing the cerebral infarction volume to increase the mRNA expressions of monoamine transmitters of 5-HT, DA, NE and 5-HIAA of corpus striatum of rats.

Key words: Naoluo Xintong decoction, ischemia reperfusion injury, regional cerebral blood flow, 5-hydroxytryptamine, dopamine, norepinephrine, 5-hydroxyindoleacetic acid rats

CLC Number: 

  • R285.5