主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2015, Vol. 38 ›› Issue (12): 852-856.doi: 10.3969/j.issn.1006-2157.2015.12.012

Previous Articles     Next Articles

Anti-obesity effects of electroacupuncture on rats with diet-induced obesity and its mechanism*

QU Yating1, LIU Xia2, HE Junfeng2,3#, LIU Zhijun2, PU Qingyang1, GUO Shengtong2, DU Jia2, JIANG Pengfei2   

  1. 1 Xiangxing College, Hunan University of Traditional Chinese Medicine, China, 410007;
    2. Institute of TCM Diagnostics, Hunan University of Traditional Chinese Medicine, Hunan;
    3 Liuyang Municipal Hospital of Traditional Chinese Medicine
  • Received:2015-07-07 Online:2015-12-30 Published:2015-12-30

Abstract: Objective To observe the effects of electroacupuncture (EA) on body weight, insulin resistance (IR), hypothalamic protein tyrosine phosphatase 1B (PTP1B) and insulin receptor substrate 1 (IRS1) in diet-induced obesity (DIO) rats, as well as its mechanism. Methods 50 SD male rats were randomly divided into low-fat diet (LFD) group (n=10) and high-fat diet (HFD) group (n=40). When the DIO model was successfully established in HFD group, DIO rats were randomly divided into model group, EA group, and Orlistat (OLST) group (n=10 in each group). EA was applied to Zusanli (ST36) and Quchi (LI11) and the stimulation was retained for 20 minutes each time for 28 consecutive days in the EA group. Rats in OLST group was orally administered with Orlistat by daily intragastric gavage (32.4 mg/kg). No intervention was offered to rats in LFD and HFD groups. After 4 weeks, all rats were sacrificed to measure the levels of fasting plasma glucose (FPG) using biochemistry technique, fasting insulin (FINS) using radioimmunoassay, PTP1B and IRS1 using Western blotting assay and morphological changes of adipocytes using HE staining. Results Body weights, levels of FPG, FINS, HOMA-IR and diameter of adipocyte in rats of EA and OLST groups were all significantly lower than those in HFD group (P<0.05). But there were no significant difference between EA, OLST and HFD groups in IRS1 (P>0.05). Rats in OLST group showed watery stools, decreased activity, mental fatigue and withered fur, while those in EA group had regular stools and lustrous fur. Conclusion EA exhibits an anti-obesity effect, probably by inhibiting the expression of PTP1B, relieving IR, reducing the weight and shrinking adipocyte, which may be the mechanism for losing weight. EA seems to be superior to Orlistat as anti-obesity therapy with less side effects.

Key words: electroacupuncture, body weight, insulin resistance, adipocyte, protein tyrosine phosphatase 1B, rats

CLC Number: 

  • R245.3