主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (1): 10-15.doi: 10.3969/j.issn.1006-2157.2016.01.002

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Lishi Huoxue formula to protect the vascular endothelial cells against hyperuricemia in rats*

LIU Chang1, WANG Chun1, ZHENG Yuan2, WANG Linyuan1, LI Wei1, ZHANG Jianjun1   

  1. 1 School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029;
    2 Xi’an Honghui Hospital
  • Received:2015-07-20 Online:2016-01-30 Published:2016-01-30

Abstract: Objective To explore the effects and mechanism of Lishi Huoxue (damp-dispersing and blood-activating) formula and two decomposed recipes Chinese herbal compound, on the vascular endothelial cells by detecting the serum levels of functional indicators of vascular endothelial cells and the aortic morphology of the rat model of hyperuricemia. Methods Male SD rats were randomly divided into seven groups: normal group, model group, benzbromarone group, Simiao Pill group, Lishi Huoxue formula (LSHX) group, decomposed recipe I for clearing heat and damp (recipe I) group, decomposed recipe Ⅱ for activating blood and resolving stasis (recipe Ⅱ)group. The rat model of hyperuricemia was established by intragastrical (i.g.)administration of yeaut and adenine. After successful modeling, rats of each medication group were administered i.g. with corresponding drugs, while normal group and model group were i.g. distilled water for consecutive 14 days. Then the serum contents of uric acid (UA) and nitric oxide (NO) were detected by ultraviolet-visible spectroscopy, and endothelin (ET-1), thromboxane B2 (TXB2) and 6-keto-PGF were measured by radioimmunoassay. Besides, thoracoabdominal aorta of all rats were dissected and stained using HE and Masson. Results Compared with normal group, the serum level of UA, ET-1 and TXB2 increased significantly in model group(P<0.05) and level of NO and 6-keto-PGF decreased(P<0.05). Compared with the model group, the serum contents of UA, ET-1 and TXB2 showed significantly decreasing in LSHX group(P<0.05),while in recipe I group UA and ET-1 decreased(P<0.05)and serum contents of NO, 6-keto-PGF in LSHX group, recipe Ⅰand Ⅱ groups increased (P<0.05). Furthermore, compared with LSHX group, the levels of TXB2 in both recipe groups and ET-1 in recipe Ⅱ group showed on statistical difference. Although LSHX and the two partial prescriptions (recipe I, II) protected against the injury of aorta, the whole prescription (LSHX) was more effective than the two decomposed recipes. Conclusion LSHX formula could reduce the serum UA, and protect vascular endothelium and the aorta of rats with hyperuricemia.

Key words: hyperuricemia, uric acid, nitric oxide, endothelin , thromboxane B2, 6-keto-PGF, aortic morphology, Lishi Huoxue formula, rats

CLC Number: 

  • R285.5