主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (1): 30-34.doi: 10.3969/j.issn.1006-2157.2016.01.006

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Influences of Ziyin Xifeng Fang on the JAK/STAT signal pathway in the kidney of spontaneously hypertensive rats*

WANG Junyan, ZHANG Lin#, JIA Lianqun, CHEN Wenna, SUN Hongwei, SHAO Chuqian, YANG Guanlin   

  1. Liaoning University of Traditional Chinese Medicine, Liaoning 110032
  • Received:2015-08-09 Online:2016-01-30 Published:2016-01-30

Abstract: Objective To observe the effects of Ziyin Xifeng Fang (ZYXFF), a Chinese medicine compound formulated by the method of nourishing yin and extinguishing wind, on the blood pressure and renal JAK/STAT signal pathway in the rat model of spontaneously hypertension (SH). Methods 40 SH rats were randomly divided into four groups (n=10 each): model group, low dose of ZYXFF(low-dose) group, high dose of ZYXFF (high-dose) group and compound apocynum group. Another 10 SKY rats with the same background were included in the control group. The rats of three treatment groups were administered intragastrically (i.g.) proper drugs, and those of control group and model group were i.g. saline for consecutive 42 days. The systolic blood pressure (BP) was measured by using small animal noninvasive blood pressure meter at Day 0, 7, 14, 21, 28, 35 and Day 42. Two hours after i.g. at the last day of medication, all the rats were sacrificed. The serum contents of creatinin (Cr), blood urea nitrogen (BUN), and uric acid (UA) were determined. The serum contents of IL-6 and TNF-α were detected by ELISA assay, and the protein expression of renal p-JAK1 and p-STAT1 were measured by Western blot. Results Compared with control group, BP, Cr, BUN, UA, IL-6 and TNF-α increased significantly in model group, and the all indexes of the rats of the three medication groups decreased remarkably(P<0.05 or P<0.01), especially the effects of high dose of ZYXFF and compound apocynum were stronger than those of low dose ZYXFF. Compared with control group, in model group rats renal p-JAK1, p-STAT1 protein expression significantly increased (P<0.01). The protein expressions of p-JAK1 and p-STAT1 decreased in the three medication groups compared with the model group (P<0.05 or P<0.01). Conclusion The mechanism of ZYXFF lowering blood pressure and attenuating the renal function impairment should be by the way of inhibiting the activation of JAK/STAT signal pathway.

Key words: Ziyin Xifeng Fang, hypertension, kidney damage, JAK/STAT signal pathway, rats

CLC Number: 

  • R285.5