主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (1): 35-39.doi: 10.3969/j.issn.1006-2157.2016.01.007

Previous Articles     Next Articles

Effects of chrysophanol on glutamate metabolism of primarily cultured strocytes in mice*

XUE Zhanxia1, GAO Yongshan2, WU Haixia1, HAO Junrong1, SHEN Lixia1#   

  1. 1 Department of Pharmacology, Hebei North University, Hebei 075000;
    2 Department of Cardiac Surgery, the First Affiliated Hospital, Hebei North University
  • Received:2015-07-21 Online:2016-01-30 Published:2016-01-30

Abstract: Objective To study the effects of different concentration of chrysophanol on the activity and glutamate metabolism of primarily cultured astrocytes in vitro. Methods Primarily cultured mice astrocytes were treated with chrysophanol at concentration of 10, 20, 40, 60, 80, 100, 200, and 250 mg/L (chry 1~8 group) for 1 d, 2 d, and 3 d. The cell activities were measured by MTT method, mRNA expressions of glutamate/aspartate transporter (GLAST) and glutamate acid transporter 1(GLT-1) were detected by using RT-PCR, and the activities of glutamine synthetase (GS) and glutathione peroxidase (GSH-Px) were also determined. Results Time-dependence and concentration-dependence of the effects of chrysophanol showed on the following: activity of astrocytes inhibited, mRNA expressions of GLAST and GLT-1 upregulated, and the activities of GSH-Px and GS increased. However, the effect of chrysophanol on extra-cellular concentration of glutamate didn’t depend on the number or activity of the cells. Conclusion Chrysophanol inhibited the activity of astrocytes in mice, and its effect on glutamate metabolism was concentration- and time-dependent.

Key words: chrysophanol, astrocyte, glutamate/aspartate transporter, glutamate acid transporter 1, glutathione peroxidase, glutamine synthetase, mice

CLC Number: 

  • R285.5