主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (1): 46-50.doi: 10.3969/j.issn.1006-2157.2016.01.009

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Effects of human gut microbiota in vitro on transformation of the total tannins content of Phyllanthus emblical L. *

YANG Guanghui, WU Lingfang, ZHANG Xiaoxue, ZHU Dan, SHAO Yanyan, MAO Xin, LIANG Wenyi, CHEN Wenjing, SHI Renbing, ZHANG Lanzhen#   

  1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102
  • Received:2015-08-07 Online:2016-01-30 Published:2016-01-30

Abstract: Objective To study in vitro the effect of human intestinal bacteria on the transformation of total tannins extracted from emblic leafflower (Yuganzi, Phyllanthus emblica L.) , a usually used Tibetan herbal medicine. Methods Based on the earlier stage work of the extraction of total tannins from Phyllanthus emblica L., activated human gut microbiota was anaerobically incubated in the culture medium GAM containing the total tannins at 37℃ for 72 hours. After centrifugation and protein precipitation, the characteristics of the metabolites were determined by using HPLC/MSn and the contents of each component determined were measured by using HPLC-UV, on which the time-content curve was drawn.Results Total 13 components were determined, including four metabolites (pyrogallic acid, methyl-derivative of gallic, urolithin B, and urolithin A), four prototype components (gallic acid, corilagin, galloyl glucose, and digalloyl glucose) and five components to be determined. The accumulated contents of gallic acid and ellagic acid were the highest at Hour 12, 10-fold higher than that of the beginning; the accumulated content of corilagin was the highest at Hour 6 with twice higher of the beginning. Conclusion After the total tannins from Phyllanthus emblica L. were transformed by human gut microbiota in vitro, certain bioactive prototype components remained and antitumor components were found.

Key words: Phyllanthus emblica L., total tannins, human gut microbiota, metabolism

CLC Number: 

  • R284.2