主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (1): 51-54.doi: 10.3969/j.issn.1006-2157.2016.01.010

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Indirect competitive enzyme-linked immunosorbent assay for determination of baicalin in Gegen Qinlian Tablet produced by seven manufacturers*

LIU Shuchen1, QU Baoping1, KONG Hui1, SHAN Wenchao2, CHENG Jinjun2, ZENG Wenhao2, QU Huihua3, WANG Qingguo1, ZHAO Yan1#   

  1. 1 School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029;
    2 School of Chinese Materia Medica, Beijing University of Chinese Medicine;
    3 Center of Scientific Research, Beijing University of Chinese Medicine
  • Received:2015-04-23 Online:2016-01-30 Published:2016-01-30

Abstract: Objective To determine the contents of baicalin in Gegen Qinlian Tablet produced by seven manufacturers, a Chinese patent drug used widely in acute gastroenteritis, virus diarrhea, and bacillary dysentery, by using indirect competitive enzyme-linked immunosorbent assay (ic-ELISA). Methods The ic-ELISA method was established by using anti-baicalin monoclonal antibody (anti-BA MAb), in which the linear relation, accuracy and the recovery rate were measured. Based on the ic-ELISA method, the contents of baicalin in Gegen Qinlian Tablet from different manufacturers were determined. Results The linear range of the ic-ELISA method established was from 16.157 to 1 280 mg/L, the relative standard deviation (RSD) was≤5.1% in the intra-assay, and≤9.0% in the inter-assay, with the average recovery rate of 107.1% (RSD=4.9%). The baicalin contents in Gegen Qinlian Tablet from seven manufacturers ranged from 4.45 to 13.41 mg per tablet. Conclusion The ic-ELISA method for the baicalin determination was convenient, accurate, and repeatable, which could be applied to the quality control of traditional Chinese herbal compound containing baicalin.

Key words: indirect competitive enzyme-linked immunosorbent assay method, Gegen Qinlian tablet, baicalin, content determination

CLC Number: 

  • R284.2