主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (1): 55-60.doi: 10.3969/j.issn.1006-2157.2016.01.011

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Determination of six lactones in Mongolian medicine compound nutmeg-5 by HPLC*

ZHU Xiaoling1,2, XU Haijie1,3, DONG Yu1#, TIAN Zi1   

  1. 1 College of Pharmacy, Inner Mongolia Medical University, Inner Mongolia 010110;
    2 Department of Pharmacy ,Inner Mongolia International Mongolian Hospital;
    3 Wuhai Food and Drug Inspection and Testing Center, Inner Mongolia
  • Received:2015-07-28 Online:2016-01-30 Published:2016-01-30

Abstract: Objective To establish a simultaneous quantitative determination method for six lactones (santamarine, costunolide, dehydrocostuslactone, alantolatone, isoalantolactone and 11,13-dihydroisoalantolantone) from nutmeg-5, a Mongolian medicine compound. Methods HPLC analysis was performed by an Elite C18 column(4.6 mm × 250 mm,5 μm) at temperature 30℃ with gradient elution (acetonitrile and aqueous solution) at a flow rate of 0.8 mL/min 204 nm. Results The six lactones were separated completedly by using the established HPLC method, and the regression equation of each lactone showed good linear correlation between concentration and peak area with the correlation coefficient more than 0.999. Each average recovery rate with relative standard deviation (RSD) of six lactones was listed as the following: santamarine 102.54% (RSD 1.02%), costunolide 103.35% (RSD 0.82%), dehydrocostuslactone 100.38% (RSD 1.75%), alantolatone 97.81% (RSD 1.59%), isoalantolactone 96.65% (RSD 1.98%), and 11,13-dihydroisoalantolantone 99.64% (RSD 1.68%). Conclusion The HPLC method was validated and found to be specific, linear, precise, and accurate. This chromatographic system can be readily used on a routine way for determination of lactones from nutmeg-5.

Key words: Mongolian medicine nutmeg-5, santamarine, costunolide, dehydrocostuslactone, alantolatone, isoalantolactone, 11,13-dihydroisoalantolantone, HPLC

CLC Number: 

  • R284.2