主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (1): 67-72.doi: 10.3969/j.issn.1006-2157.2016.01.013

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Optimization extraction of Sophora alopecurcurodies based on the anti-inflammatory effect experiment combined with orthogonal test*

LI Hui, MA Shuwei, LIU Yan, RU Qingguo, LIU Jia, CHEN Yanyan, WU Qing#   

  1. School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100102
  • Received:2015-04-27 Online:2016-01-30 Published:2016-01-30

Abstract: Objective To optimize the procedure of extracting components from Sophora alopecurcurodies (Kudouzi). Methods After external use of the extracts of Kudouzi by four different extraction procedures on the footpad inflammation rat model induced by carrageenan, the swelling rate was observed as the therapeutic index. The contents of oxysop hocarpine and oxymatrine of extracts from Kudouzi, as the two chemical indexes, were measured by using HPLC. The extraction procedure was screened by therapeutic index combined with chemical indexes. Then the parameters of the procedure screened were optimized by orthogonal test. Results All the extracts inhibited footpad swelling in rats, and the effect of the ethanol extract was the strongest, and in which the contents of oxysop hocarpine and oxymatrine were the highest. The footpad swelling rate was negatively correlated with the contents of the two chemical indexes. The optimized extraction procedure was obtained with 10 times of 80%(V/V) alcohol, 4 hours at 50℃. Conclusion The extraction procedure of Kudouzi was stable and feasible, and the contents of active components were positively correlated with their effects.

Key words: Sophora alopecurcurodies, extraction procedure, therapeutic indexes, chemical components , orthogonal test

CLC Number: 

  • R283