主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (2): 122-127.doi: 10.3969/j.issn.1006-2157.2016.02.007

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Toll-like receptor-8 gene rs3764880 polymorphism and ischemic stroke risk in patients of Han Chinese males*

GU Lian1, ZHOU Jinying2, TAN Jinjing3, LIANG Baoyun1, YAN yan1, CHEN Qing1, XIE Juanjuan3, TANG Nong2#   

  1. 1 Department of Neurology, First Affiliated Hospital, Guangxi University of Traditional Chinese Medicine, Guangxi 530023;
    2 Guangxi University of Traditional Chinese Medicine;
    3 Department of Intensive Medicine, the First Affiliated Hospital, Guangxi University of Traditional Chinese Medicine
  • Received:2015-08-17 Online:2016-02-28 Published:2016-02-28

Abstract: Objective To explore the association between Toll-like receptor-8(TLR8) genetic polymorphisms and susceptibility to ischemic stroke (IS) in patients of different traditional Chinese medicine (TCM) patterns. Methods 311 IS patients of wind-phlegm stasis-obstruction pattern, 284 IS patients of qi-deficiency blood-stasis pattern and 605 controls were recruited. The real-time PCR technology was used to detect TLR8 gene expression levels. TLR8 rs3764880 single nucleotide polymorphism(SNPs) were genotyped using the Sequenom Mass ARRAY platform. Levels of inflammatory cytokines were measured by using ELISA. Statistical analyses were carried out by using PLINK and SPSS 16.0 software. Results TLR8 gene variant rs3764880 polymorphism was significantly associated with wind-phlegm stasis-obstruction pattern in IS males patients in the allele model (P<0.05). In IS male patients of qi-deficiency blood-stasis pattern , level of IL-8 was higher in the GG genotype carriers than the AA+AG genotype carriers (P<0.05). The GG+AG genotype carriers had higher levels of TNF-α and IL-8 than the AA genotype carriers(P<0.05) . TLR8 gene variant rs3764880 polymorphism was significantly associated with the levels of TC (βb=-0.03, Padj=0.001) and LDL (βb=-0.25, Padj=0.018) in additive model in qi-deficiency blood-stasis pattern in Han Chinese IS male patients. Conclusion TLR8 gene rs3764880 polymorphism might be associated with the susceptibility to IS in patients of wind-phlegm stasis-obstruction and affect inflammatory reaction and lipid metabolism in Han Chinese males of qi-deficiency blood-stasis pattern.

Key words: ischemic stroke, traditional Chinese medicine pattern, Toll-like receptor-8, single nucleotide polymorphism, inflammatory cytokines

CLC Number: 

  • R255.2