主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (2): 151-154.doi: 10.3969/j.issn.1006-2157.2016.02.013

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Optimization of extraction technology for total saponins from Rhizoma Panacis Majoris using orthogonal test *

LI Fengying, WANG Yingzi#, WANG Qing, ZHANG Xiuting, ZHANG Chunni, LI Wenhua, LUO Shengxiu   

  1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102
  • Received:2015-07-17 Online:2016-02-28 Published:2016-02-28

Abstract: Objective To optimize extraction technology for total saponins from Rhizoma Panacis Majoris(zhuzishen) and provide a theoretical basis for development and utilization of saponins from Rhizoma Panacis Majoris. Methods Ultraviolet spectrophotometry was employed to detect content of total saponins from Rhizoma Panacis Majoris with detection wave length set at 540 nm. And effects of ethanol concentration, solvent quantity, extraction time and extraction times on extraction process were investigated by using orthogonal test design. Results Linear range of total saponins from Rhizoma Panacis Majoris was 0.052~0.182 mg, and the average recovery rate was 98.64%. The best extraction technology for total saponins from Rhizoma Panacis Majoris was to extract 3 times with 10 times treatment of 70% ethanol for 1 h per time. The average yield rate of total saponins from Rhizoma Panacis Majoris was 12.32%. Conclusion This established ultraviolet spectrophotometry was accurate and reliable to detect contents of total saponins from Rhizoma Panacis Majoris. At the same time, this optimized extraction technology of total saponins from Rhizoma Panacis Majoris was simple, stable and feasible.

Key words: Rhizoma Panacis Majoris, total saponin, extract technology, orthogonal test

CLC Number: 

  • R284.2