主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (2): 162-168.doi: 10.3969/j.issn.1006-2157.2016.02.015

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Studies on the serum pharmacochemistry of Yinchenhao Decoction*

XU Bo1, DOU Zhihua2#, LUO Lin3, GU Wei2, MENG Ping2, LIU Qingqing2, WANG Zhengyu2   

  1. 1 College of Pharmacy, Nanjing University of Chinese Medicine, Jiangsu 210023;
    2 Nantong Clinical College of Integrated Traditional Chinese Medicine and Western Medicine, Nanjing University of Chinese Medicine;
    3 College of Pharmacy, Nantong University
  • Received:2015-07-28 Online:2016-02-28 Published:2016-02-28

Abstract: Objective To analyze the constituents of Yinchenhao Decoction (YCHD) absorbed into blood by serum pharmacochemistry. Methods SD rats were used as donor and serum containing drugs were prepared after intragastric administration of YCHD, YCHD without one of the component herbs, and individual herbs of YCHD. HPLC fingerprints of serum containing drug were established. By comparing the fingerprints of serum containing YCHD with the one of control serum, drug-induced constituents in blood were distinguished. By comparing the fingerprint of serum containing YCHD with the ones of serum containing YCHD without either one of the component herbs, and individual herbs of YCHD, the sources of drug-induced constituents were analyzed. By comparing the retention time and spectrograms of chromatographic peaks in fingerprint of serum containing YCHD with the ones of YCHD and reference substances, drug-induced constituents in blood were identified. Results 23 drug-induced constituents in serum containing YCHD were detected. 6 of them were original compounds, other 17 were metabolites. 3 constituents out of 6 original compounds were identified as geniposidic acid and geniposide (iridoids from Gardeniae Fructus), and rhein (anthraquinone from Rhei Radix et Rhizoma), other three original compounds were identified as anthraquinone from Rhei Radix et Rhizoma(RRR) and 2 crocetin derivatives from Gardeniae Fructus(GF). Out of 17 metabolites, 1 was identified as the metabolite of iridoid, 7 were identified as the metabolites of anthraquinone, and 9 were identified as the metabolites of crocetin derivatives. Conclusion Drug-induced constituents in serum containing YCHD include original form of anthraquinones from RRR, original form of iridoids and crocetin derivatives from GF, and the metabolites of the constituents mentioned above. All these drug-induced constituents may be the pharmacodynamic material basis for hepatoprotective effects of YCHD.

Key words: Yinchenhao Decoction, Artemisiae Scopariae Herba, Gardeniae Fructus, Rhei Radix et Rhizoma, serum containing drug, serum pharmacochemistry, fingerprint

CLC Number: 

  • R284.1