主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (3): 230-234.doi: 10.3969/j.issn.1006-2157.2016.03.011

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Effects of curcumin on β-amyloid generating enzyme and degrading enzymes in hippocampus in mice*

DONG Yunfang1, FENG Huili1, HE Yingkun1,FAN Hui1,2,DANG Huizi1,3, REN Ying1, WANG Pengwen1#   

  1. 1 Key Laboratory of Chinese Internal Medicine, Beijing University of Chinese Medicine, Ministry of Education, Beijing 100700;
    2 Beijing Chaoyang Combined Traditional Chinese and Western Medicine Emergency Medical Center;
    3 Beijing Hui People Hospital
  • Received:2015-10-20 Online:2016-03-30 Published:2016-03-30

Abstract: Objective To explore the sustainable effects of curcumin on presenilin 2(PS2), β-amyloid (Aβ) generation enzyme, and insulin degrading enzyme (IDE) and neprilysin (NEP), Aβ degrading enzymes, in hippocampal CA1 region of 9-month-old Alzheimer’s disease (AD)mice. Methods The 3-month-old APP/PS1 double transgenic mice were randomly divided into model group, rosiglitazone group, curcumin high-dose, mid-dose and low-dose group at dose of 400, 200, 100 mg/kg, with littermate non-transgenic mice involved in the control group. After intragastrical administration medicines for consecutive six months, expressions of PS2, IDE and NEP were detected using imunohistochemistry and Western-blot methods. Results Compared with the control group, expression of PS2 increased in the model group significantly(P<0.01), and compared with the model group, expression of PS2 decreased in all the intervention groups (P<0.01 or P<0.05)with PS2-positive cells in hippocampus CA1 region decreasing (P<0.05). Compared with the control group, expressions of IDE and NEP decreased significantly in the model group (P<0.01); compared with the model group, expressions of IDE and NEP increased significantly in all the intervention groups with IDE and NEP-positive cells in hippocampus CA1 region increasing(P<0.01 or P<0.05). Conclusion It is suggested that one of potential mechanisms of curcumin reducing Aβ accumulation may be via increasing Aβ-degrading enzyme IDE and NEP expression and reducing Aβ-generating enzyme PS2 expression.

Key words: curcumin, Alzheimer’s disease, presenilin 2, insulin degrading enzyme, neprilysin, mice

CLC Number: 

  • R285.5