主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (3): 241-246.doi: 10.3969/j.issn.1006-2157.2016.03.013

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Effects of acupuncture on cysteine-containing aspartate-specific proteases 3 and c-Jun in the hippocampus of depression rats induced by force swimming stress*

GUO Yu1, BAO Wuye2, TU Ya1#,WU Jihong1,WANG Yu1, ZHANG Xuhui1, XU Mingmin1, YU Miao1, ZHANG Chuntao1, ZHAO Bingcong1   

  1. 1 School of Acupuncture-Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 100029;
    2 Affiliated Hospital of Inner Mongolia University for the Nationalities
  • Received:2015-10-06 Online:2016-03-30 Published:2016-03-30

Abstract: Objective To observe the interventional effect of acupuncture on apoptosis of hippocampal neuron of depression model rats, and the effect on the c-Jun N-terminal kinase(JNK)pathway, so as to reveal the mechanism of anti-depression of acupuncture. Methods Altogether 36 Sprague-Dawley rats were randomly divided into six groups (each n=6): control group, control+ inhibitor (SP600125, a JNK inhibitor ) group, model group, model + inhibitor group, acupuncture group and acupuncture + inhibitor group. Except the control group and control + inhibitor group, the rats in the other four groups were forced to swim and be bred solitarily to establish the rat depression model. Baihui (GV20) and Yintang (GV29), the acupoints selected in rats of acupuncture group and acupuncture + inhibitor group, were needled for 20 minutes, once daily for two days. Using Western blot to detect the protein expression of c-Jun, the substrate of JNK pathway, in hippocampus of all groups of rats, and using RT-PCR to examine the gene expression of apoptosis-related caspase-3 in hippocampus of rats. Results Compared with the control group, caspase-3 gene expression and c-Jun protein expression showed no significant difference in control + inhibitor group(P>0.05), but both were higher in those of model group(P<0.01). Compared with model group, the expression levels of caspase-3 were lower significantly in model+inhibitor group, acupuncture group and acupuncture + inhibitor group(P<0.01), and the protein expression levels of c-Jun were lower significantly in model+inhibitor group, acupuncture group and acupuncture + inhibitor group(P<0.05,P<0.05,P<0.01). Conclusion Apoptosis of hippocampal neurons may be inhibited via the interventional effect of acupuncture on JNK pathway activities which down-regulate c-Jun protein expression and apoptosis-related caspase-3 gene expression. It may be one of the potential molecular mechanisms of antidepressant effect of acupuncture.

Key words: acupuncture, forced swimming stress, hippocampus, depression, cysteine-containing aspartate-specific protease 3, c-Jun, JNK inhibitor SP600125, rats

CLC Number: 

  • R245.31+1