主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (4): 298-303.doi: 10.3969/j.issn.1006-2157.2016.04.007

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Components in Radix Aconiti before and after processing by using HPLC-MS*

QIN Yuxin, ZHANG Xianling, WANG Lei, ZHANG Yufan, WANG Xin, TAN Peng, LI Fei#   

  1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102
  • Received:2015-11-20 Online:2016-04-30 Published:2016-04-30

Abstract: Objective To contrast the components in Radix Aconiti (Common Monkshood Mother Root, Chuanwutou) before and after processing, so as to provide primary evidence for interpreting the processing principles of Radix Aconiti. Methods HPLC analysis was performed on Thermo SCIENTIFIC high performance liquid chromatograph, with Agilent Extend-C18 column eluted with acetonitrile and 0.2% ammonia in a gradient program at wavelength 240 nm, and mass spectrum(MS) method was conducted in the positive ion mode. Results Total 99 components were detected in unprocessed Radix Aconiti, and 106 components were included in Radix Aconiti Praeparata. There were 53 in common. After processing, 46 components disappeared with 53 more were detected. And 64 components were identified in unprocessed Radix Aconiti. Conclusion The change of components of Radix Aconiti before and after processing was proved by the method of HPLC-MS, which was more effective and comprehensive than the method of HPLC detecting six alkaloids.

Key words: Radix Aconiti, processing, HPLC-MS, component

CLC Number: 

  • R284.1