主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (4): 308-315.doi: 10.3969/j.issn.1006-2157.2016.04.009

Previous Articles     Next Articles

Quality representation and correlation analysis of Aurea Helianthus based on characteristic chromatogram of drug system*

FENG Duo1, TAN Li1, PENG Ping1, SHEN Lifeng2, XU Shuya1, LV Shan1, ZHANG Yuanyuan1, CHEN Wei1, ZHANG Fang1, SHI Renbing1,3#   

  1. 1 Key Unit of Exploring Effective Substances of Classical and Famous Formulas of State Administration of Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102;
    2 School of Traditional Chinese Medicine, Capital Medical University;
    3 Quality Control Technology and Engineering Center of Chinese Medicine, Beijing Municipal Commission of Education
  • Received:2015-11-20 Online:2016-04-30 Published:2016-04-30

Abstract: Objective To establish quality representation and correlation analysis method based on the characteristic chromatograms of drug system of Aurea helianthus (Jinhuakui), so as to evaluate quality of Aurea helianthus effectively and accurately. Methods Using HPLC-PDA method to characterize the 19 characteristic peaks and their chemical types in specific chromatograms of Aurea helianthus to represent the quality of altoghther 15 batches of Aurea helianthus. The quantitative representation of the 15 batches of Aurea helianthus was based on the contents of 5 characteristic index component-chlorogenic acid, rutinum, hyperoside, isoquercitrin, quercetin, phenylpropionic acids (represented by chlorogenic acid) and flavonoids ( represented by hyperoside). Correlation analysis of quality and quantity representations was then performed based on reference. Results Total 19 characteristic peaks (3 peaks of phenylpropionic acids and 16 peaks of flavonoids) appeared in the chromatogram of all the 15 batches. By taking Batch 1 as the reference, the total contents of the effective index components of Batch 10, 14, 8, 11, 6 and 5 were higher than the reference, and the highest correlation with the reference showed in Batch 5, 6, 3, 7, 2, 11 and 12. So the quality of Batch 5, 6, 11, 3, 7 and 2 were superio to the others. Conclusion The quality characterization correlation analysis model established for Aurea helianthus, concerning on systemic correlation and application validity, evaluated the quality of Aurea helianthus effectively and accurately.

Key words: Aurea helianthus, specific chromatogram, correlation analysis, chlorogenic acid, rutinum, hyperoside, isoquercitrin, quercetin

CLC Number: 

  • R284.1